LOCUS       sequence-165363-        3981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing SETDB1, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SETDB1_WT
FEATURES             Location/Qualifiers
     source          1..3981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      35..623
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     524..543
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     777..794
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(953..980)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1072..1158)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1207..1229
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1221..1238
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1222..1238
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1254..1353
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2545..2644)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2661..2680)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2662..2680)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2685..2701)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2685..2701)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3132..3923
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
       61 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
      121 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
      181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      541 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
      601 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
      661 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
      721 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
      781 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
      841 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
      901 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
      961 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     1021 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     1081 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     1141 agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga
     1201 tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg ccccaaataa
     1261 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt
     1321 ttataatgcc aactttgtac aaaaaagttg gcatgtcttc ccttcctggg tgcattggtt
     1381 tggatgcagc aacagctaca gtggagtctg aagagattgc agagctgcaa caggcagtgg
     1441 ttgaggaact gggtatctct atggaggaac ttcggcattt catcgatgag gaactggaga
     1501 agatggattg tgtacagcaa cgcaagaagc agctagcaga gttagagaca tgggtaatac
     1561 agaaagaatc tgaggtggct cacgttgacc aactctttga tgatgcatcc agggcagtga
     1621 ctaattgtga gtctttggtg aaggacttct actccaagct gggactacaa taccgggaca
     1681 gtagctctga ggacgaatct tcccggccta cagaaataat tgagattcct gatgaagatg
     1741 atgatgtcct cagtattgat tcaggtgatg ctgggagcag aactccaaaa gaccagaagc
     1801 tccgtgaagc tatggctgcc ttaagaaagt cagctcaaga tgttcagaag ttcatggatg
     1861 ctgtcaacaa gaagagcagt tcccaggatc tgcataaagg aaccttgagt cagatgtctg
     1921 gagaactaag caaagatggt gacctgatag tcagcatgcg aattctgggc aagaagagaa
     1981 ctaagacttg gcacaaaggc acccttattg ccatccagac agttgggcca gggaagaaat
     2041 acaaggtgaa atttgacaac aaaggaaaga gtctactgtc ggggaaccat attgcctatg
     2101 attaccaccc tcctgctgac aagctgtatg tgggcagtcg ggtggtcgcc aaatacaaag
     2161 atgggaatca ggtctggctc tatgctggca ttgtagctga gacaccaaac gtcaaaaaca
     2221 agctcaggtt tctcattttc tttgatgatg gctatgcttc ctatgtcaca cagtcggaac
     2281 tgtatcccat ttgccggcca ctgaaaaaga cttgggagga catagaagac atctcctgcc
     2341 gtgacttcat agaggagtat gtcactgcct accccaaccg ccccatggta ctgctcaaga
     2401 gtggccagct tatcaagact gagtgggaag gcacgtggtg gaagtcccga gttgaggagg
     2461 tggatggcag cctagtcagg atcctcttcc tggtactgtt cttctctaca attttggagg
     2521 cagaggttgg tgggggggga acttacccaa ctttcttgta caaagttggc attataagaa
     2581 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     2641 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
     2701 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     2761 catcatgcct cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg
     2821 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct
     2881 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     2941 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     3001 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     3061 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     3121 agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     3181 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     3241 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     3301 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     3361 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     3421 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     3481 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     3541 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     3601 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     3661 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     3721 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     3781 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     3841 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
     3901 tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga
     3961 gttacgcgtc gttccactga g
//
