LOCUS       sequence-165364-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into MFE1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Schwartz C, Shabbir-Hussain M, Frogue K, Blenner M, Wheeldon I
  TITLE     Standardized markerless gene integration for pathway engineering in 
            Yarrowia lipolytica.
  JOURNAL   ACS Synth Biol. 2016 Dec 19.
  PUBMED    27989123
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Dec 19."
COMMENT     Sequence Label: pHR_MFE1_hrGFP
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..716)
                     /codon_start=1
                     /product="humanized Renilla green fluorescent protein"
                     /label=hrGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFGN
                     QLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLRYE
                     DGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGVLVG
                     QVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQHETAI
                     AQLTSLGKPLGSLHEW"
ORIGIN
        1 acccactcgt gcaggctgcc caggggcttg cccaggctgg tcagctgggc gatggcggtc
       61 tcgtgctgct ccacgaagcc gccgtcctcc acgtaggtct tctccaggcg gtgctggatg
      121 aagtggtact cggggaagtc cttcaccacg cccttgctct tcatcagggt gcgcatgtgg
      181 cagctgtaga acttgccgct gttcaggcgg tacaccagga tcacctggcc caccagcacg
      241 ccgtcgttca tgtacaccac ctcgaagctg ggctgcaggc cggtgatggt cttcttcatc
      301 acggggccgt cgttggggaa gttgcggccc ttgtactcca cgcggtacac gaacatctcc
      361 tcgatcaggt tgatgtcgct gcggatctcc accaggccgc cgtcctcgta gcgcagggtg
      421 cgctcgtaca cgaagccggc ggggaagctc tggatgaaga agtcgctgat gtcctcgggg
      481 tacttggtga aggtgcggtt gccgtactgg aaggcggggc tcaggatgtc gaaggcgaag
      541 ggcagggggg cgcccttggt cacgcggatc tgcaccagct ggttgccgaa caggatgttg
      601 cccttgccgc agccctccat ggtgaacacg tggttgttca ccacgccctc caggttcacc
      661 ttgaagctca tgatctcctg caggccggtg ttcttcagga tctgcttgct caccatggcg
      721 cgcctttgaa tgattcttat actcagaagg aaatgcttaa cgatttcggg tgtgagttga
      781 caaggagaga gagaaaagaa gaggaaaggt aattcgggga cggtggtctt ttataccctt
      841 ggctaaagtc ccaaccacaa aagcttctag gctgcaggca tgcccgga
//
