LOCUS       sequence-165370-         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, 3x-HA-GFP to a 
            3xT7-RFP tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Menendez-Benito V, van Deventer SJ, Jimenez-Garcia V, Roy-Luzarraga 
            M, van Leeuwen F, Neefjes J
  TITLE     Spatiotemporal analysis of organelle and macromolecular complex 
            inheritance.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jan 2;110(1):175-80. doi: 
            10.1073/pnas.1207424110. Epub 2012 Dec 17.
  PUBMED    23248297
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1207424110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-01-2- 2"; volume: "110"; issue: "1"; pages: "175-80"
COMMENT     Sequence Label: pKV016
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..99
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             108..134
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             141..167
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             171..197
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 actcactata gggcgaattg ggcccaatgc attggcgccg cggccgcggt ggatctggtg
       61 gatctataac ttcgtataat gtatgctata cgaagttatc aggtacctac ccatacgatg
      121 ttcctgacta tgcgactagc tatccctatg acgtcccgga ctatgcagga tatccatatg
      181 acgttccaga ttacgctact agtatgtcta aaggtgaaga attattcact ggtgttgtcc
      241 caattttggt tgaattagat ggtgatgtta atggtcacaa attttctgtc tccggtgaag
      301 gtgaaggtga tgctacttac ggtaaattga ccttaaaatt tatttgtact actggtaaat
      361 tgccagttcc atggccaacc ttagtcacta ctttaactta tggtgttcaa tgtttttcta
      421 gatacccaga tcatatgaaa caacatgact ttttcaagtc tgccatgcca gaaggttatg
      481 ttcaagaaag aactattttt ttcaaagatg acggtaacta caagaccaga gctgaagtca
      541 agtttgaagg tgatacctta gttaatagaa tcgaattaaa aggtattgat tttaaagaag
      601 atggtaacat tttaggtcac aaattggaat acaactataa ctctcacaat gtttacatca
      661 tggctgacaa acaaaagaat ggtatcaaag ttaacttcaa aattagacac aacattgaag
      721 atggttctgt tcaattagct gaccattatc aacaaaatac tccaattggt gatggtccag
      781 tcttgttacc agacaaccat tacttatcca ctcaatctgc cttatccaaa gatccaaacg
      841 aaaagagaga ccacatggtc ttgttagaat ttgttactgc tgctggtatt acc
//
