LOCUS       sequence-165371-        4305 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ACVR1B, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_ACVR1B_WT
FEATURES             Location/Qualifiers
     source          1..4305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(108..135)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(227..313)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     362..384
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     376..393
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     377..393
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    409..508
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2024..2123)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2140..2159)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2141..2159)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2164..2180)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2164..2180)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2611..3402
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3495..4083
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3984..4003
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4237..4254
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
       61 gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg
      121 tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc
      181 accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca
      241 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct
      301 ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt
      361 tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggcccca aataatgatt
      421 ttattttgac tgatagtgac ctgttcgttg caacaaattg atgagcaatg cttttttata
      481 atgccaactt tgtacaaaaa agttggcatg gcggagtcgg ccggagcctc ctccttcttc
      541 ccccttgttg tcctcctgct cgccggcagc ggcgggtccg ggccccgggg ggtccaggct
      601 ctgctgtgtg cgtgcaccag ctgcctccag gccaactaca cgtgtgagac agatggggcc
      661 tgcatggttt ccattttcaa tctggatggg atggagcacc atgtgcgcac ctgcatcccc
      721 aaagtggagc tggtccctgc cgggaagccc ttctactgcc tgagctcgga ggacctgcgc
      781 aacacccact gctgctacac tgactactgc aacaggatcg acttgagggt gcccagtggt
      841 cacctcaagg agcctgagca cccgtccatg tggggcccgg tggagctggt aggcatcatc
      901 gccggcccgg tgttcctcct gttcctcatc atcatcattg ttttccttgt cattaactat
      961 catcagcgtg tctatcacaa ccgccagaga ctggacatgg aagatccctc atgtgagatg
     1021 tgtctctcca aagacaagac gctccaggat cttgtctacg atctctccac ctcagggtct
     1081 ggctcagggt tacccctctt tgtccagcgc acagtggccc gaaccatcgt tttacaagag
     1141 attattggca agggtcggtt tggggaagta tggcggggcc gctggagggg tggtgatgtg
     1201 gctgtgaaaa tattctcttc tcgtgaagaa cggtcttggt tcagggaagc agagatatac
     1261 cagacggtca tgctgcgcca tgaaaacatc cttggattta ttgctgctga caataaagat
     1321 aatggcacct ggacacagct gtggcttgtt tctgactatc atgagcacgg gtccctgttt
     1381 gattatctga accggtacac agtgacaatt gaggggatga ttaagctggc cttgtctgct
     1441 gctagtgggc tggcacacct gcacatggag atcgtgggca cccaagggaa gcctggaatt
     1501 gctcatcgag acttaaagtc aaagaacatt ctggtgaaga aaaatggcat gtgtgccata
     1561 gcagacctgg gcctggctgt ccgtcatgat gcagtcactg acaccattga cattgccccg
     1621 aatcagaggg tggggaccaa acgatacatg gcccctgaag tacttgatga aaccattaat
     1681 atgaaacact ttgactcctt taaatgtgct gatatttatg ccctcgggct tgtatattgg
     1741 gagattgctc gaagatgcaa ttctggagga gtccatgaag aatatcagct gccatattac
     1801 gacttagtgc cctctgaccc ttccattgag gaaatgcgaa aggttgtatg tgatcagaag
     1861 ctgcgtccca acatccccaa ctggtggcag agttatgagg cactgcgggt gatggggaag
     1921 atgatgcgag agtgttggta tgccaacggc gcagcccgcc tgacggccct gcgcatcaag
     1981 aagaccctct cccagctcag cgtgcaggaa gacgtgaaga tctacccaac tttcttgtac
     2041 aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc
     2101 agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac
     2161 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt
     2221 gcacaagata aaaatatatc atcatgcctc ctctagacca gccaggacag aaatgcctcg
     2281 acttcgctgc tacccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga
     2341 acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca
     2401 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
     2461 atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa
     2521 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
     2581 ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc cgaagtatcg
     2641 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc
     2701 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg
     2761 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt
     2821 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt
     2881 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga
     2941 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat
     3001 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg
     3061 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta
     3121 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg
     3181 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac
     3241 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct
     3301 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
     3361 cactacgtga aaggcgagat caccaaggta gtcggcaaat aaccctcgag ccacccatga
     3421 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     3481 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3541 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3601 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     3661 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3721 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3781 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3841 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3901 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3961 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     4021 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     4081 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     4141 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     4201 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     4261 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccg
//
