LOCUS       sequence-165376-        3684 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CDK2, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CDK2_WT
FEATURES             Location/Qualifiers
     source          1..3684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     222..241
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     475..492
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(651..678)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(770..856)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     905..927
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     919..936
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     920..936
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    952..1051
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1946..2045)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2062..2081)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2063..2081)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2086..2102)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2086..2102)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2533..3324
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3417..3684,1..321)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
       61 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
      121 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
      181 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
      241 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
      301 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
      361 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
      421 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
      481 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
      541 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
      601 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat
      661 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc
      721 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac
      781 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag
      841 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg
      901 ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc ccaaataatg
      961 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt
     1021 ataatgccaa ctttgtacaa aaaagttggc atggagaact tccaaaaggt ggaaaagatc
     1081 ggagagggca cgtacggagt tgtgtacaaa gccagaaaca agttgacggg agaggtggtg
     1141 gcgcttaaga aaatccgcct ggacactgag actgagggtg tgcccagtac tgccatccga
     1201 gagatctctc tgcttaagga gcttaaccat cctaatattg tcaagctgct ggatgtcatt
     1261 cacacagaaa ataaactcta cctggttttt gaatttctgc accaagatct caagaaattc
     1321 atggatgcct ctgctctcac tggcattcct cttcccctca tcaagagcta tctgttccag
     1381 ctgctccagg gcctagcttt ctgccattct catcgggtcc tccaccgaga ccttaaacct
     1441 cagaatctgc ttattaacac agagggggcc atcaagctag cagactttgg actagccaga
     1501 gcttttggag tccctgttcg tacttacacc catgaggtgg tgaccctgtg gtaccgagct
     1561 cctgaaatcc tcctgggctg caaatattat tccacagctg tggacatctg gagcctgggc
     1621 tgcatctttg ctgagatggt gactcgccgg gccctattcc ctggagattc tgagattgac
     1681 cagctcttcc ggatctttcg gactctgggg accccagatg aggtggtgtg gccaggagtt
     1741 acttctatgc ctgattacaa gccaagtttc cccaagtggg cccggcaaga ttttagtaaa
     1801 gttgtacctc ccctggatga agatggacgg agcttgttat cgcaaatgct gcactacgac
     1861 cctaacaagc ggatttcggc caaggcagcc ctggctcacc ctttcttcca ggatgtgacc
     1921 aagccagtac cccatcttcg actctgccca actttcttgt acaaagttgg cattataaga
     1981 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
     2041 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     2101 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     2161 tcatcatgcc tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag
     2221 gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc
     2281 tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc
     2341 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
     2401 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     2461 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     2521 aagttaaaca ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     2581 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     2641 gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta
     2701 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     2761 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     2821 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     2881 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     2941 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     3001 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     3061 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     3121 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     3181 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     3241 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag
     3301 atcaccaagg tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg
     3361 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3421 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3481 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3541 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     3601 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3661 agtggcgata agtcgtgtct tacc
//
