LOCUS       sequence-165377-         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of SRC. Intended for 
            co-expression with YopH Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: SRC_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             61..83
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             103..132
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             133..153
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ggctccggag agctctttaa ttaagcggcc gccctgcagg actcgagttc tagaaataat
       61 tttgtttaac tttaagaagg agatatacat atgaaatctt ctcaccatca ccatcaccat
      121 caccatcacc atgaaaacct gtacttccaa tccaatgcac agactcaggg cctggccaag
      181 gatgcctggg agatccctcg ggagtcgctg cggctggagg tcaagctggg ccagggctgc
      241 tttggcgagg tgtggatggg gacctggaac ggtaccacca gggtggccat caaaaccctg
      301 aagcctggca cgatgtctcc agaggccttc ctgcaggagg cccaggtcat gaagaagctg
      361 aggcatgaga agctggtgca gttgtatgct gtggtttcag aggagcccat ttacatcgtc
      421 acggagtaca tgagcaaggg gagtttgctg gactttctca agggggagac aggcaagtac
      481 ctgcggctgc ctcagctggt ggacatggct gctcagatcg cctcaggcat ggcgtacgtg
      541 gagcggatga actacgtcca ccgggacctt cgtgcagcca acatcctggt gggagagaac
      601 ctggtgtgca aagtggccga ctttgggctg gctcggctca ttgaagacaa tgagtacacg
      661 gcgcggcaag gtgccaaatt ccccatcaag tggacggctc cagaagctgc cctctatggc
      721 cgcttcacca tcaagtcgga cgtgtggtcc ttcgggatcc tgctgactga gctcaccaca
      781 aagggacggg tgccctaccc tgggatggtg aaccgcgagg tgctggacca ggtggagcgg
      841 ggctaccgga tgccctgccc gccggagtgt cccgagtccc tgcacgacct catgtgccag
      901 tgctggcgga aggagcctga ggagcggccc accttcgagt acctgcaggc cttcctggag
      961 gactacttca cgtccaccga gccc
//
