LOCUS       sequence-165378-        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of SRC. Intended for 
            co-expression with YopH Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: SRC_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(962..982)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(983..1003)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatcagc gcgcacgcgt ggtaccggcc ggccaccagg
       61 tggcgcgccg cgatcgcgga tccgttatcc acttccaatg ttattagagg ttctccccgg
      121 gctggtactg gggctcggtg gacgtgaagt agtcctccag gaaggcctgc aggtactcga
      181 aggtgggccg ctcctcaggc tccttccgcc agcactggca catgaggtcg tgcagggact
      241 cgggacactc cggcgggcag ggcatccggt agccccgctc cacctggtcc agcacctcgc
      301 ggttcaccat cccagggtag ggcacccgtc cctttgtggt gagctcagtc agcaggatcc
      361 cgaaggacca cacgtccgac ttgatggtga agcggccata gagggcagct tctggagccg
      421 tccacttgat ggggaatttg gcaccttgcc gcgccgtgta ctcattgtct tcaatgagcc
      481 gagccagccc aaagtcggcc actttgcaca ccaggttctc tcccaccagg atgttggctg
      541 cacgaaggtc ccggtggacg tagttcatcc gctccacgta cgccatgcct gaggcgatct
      601 gagcagccat gtccaccagc tgaggcagcc gcaggtactt gcctgtctcc cccttgagaa
      661 agtccagcaa actccccttg ctcatgtact ccgtgacgat gtaaatgggc tcctctgaaa
      721 ccacagcata caactgcacc agcttctcat gcctcagctt cttcatgacc tgggcctcct
      781 gcaggaaggc ctctggagac atcgtgccag gcttcagggt tttgatggcc accctggtgg
      841 taccgttcca ggtccccatc cacacctcgc caaagcagcc ctggcccagc ttgacctcca
      901 gccgcagcga ctcccgaggg atctcccagg catccttggc caggccctga gtctgtgcat
      961 tggattggaa gtacaggttt tcatggtgat ggtgatggtg atg
//
