LOCUS       sequence-165379-        3699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CDK4, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CDK4_WT
FEATURES             Location/Qualifiers
     source          1..3699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     15..37
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     29..46
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     30..46
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    62..161
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1071..1170)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1187..1206)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1188..1206)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1211..1227)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1211..1227)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1658..2449
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2542..3130
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3031..3050
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3284..3301
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3460..3487)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3579..3665)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 agcatggatg ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc
       61 ccaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca
      121 atgctttttt ataatgccaa ctttgtacaa aaaagttggc atggctacct ctcgatatga
      181 gccagtggct gaaattggtg tcggtgccta tgggacagtg tacaaggccc gtgatcccca
      241 cagtggccac tttgtggccc tcaagagtgt gagagtcccc aatggaggag gaggtggagg
      301 aggccttccc atcagcacag ttcgtgaggt ggctttactg aggcgactgg aggcttttga
      361 gcatcccaat gttgtccggc tgatggacgt ctgtgccaca tcccgaactg accgggagat
      421 caaggtaacc ctggtgtttg agcatgtaga ccaggaccta aggacatatc tggacaaggc
      481 acccccacca ggcttgccag ccgaaacgat caaggatctg atgcgccagt ttctaagagg
      541 cctagatttc cttcatgcca attgcatcgt tcaccgagat ctgaagccag agaacattct
      601 ggtgacaagt ggtggaacag tcaagctggc tgactttggc ctggccagaa tctacagcta
      661 ccagatggca cttacacccg tggttgttac actctggtac cgagctcccg aagttcttct
      721 gcagtccaca tatgcaacac ctgtggacat gtggagtgtt ggctgtatct ttgcagagat
      781 gtttcgtcga aagcctctct tctgtggaaa ctctgaagcc gaccagttgg gcaaaatctt
      841 tgacctgatt gggctgcctc cagaggatga ctggcctcga gatgtatccc tgccccgtgg
      901 agcctttccc cccagagggc cccgcccagt gcagtcggtg gtacctgaga tggaggagtc
      961 gggagcacag ctgctgctgg aaatgctgac ttttaaccca cacaagcgaa tctctgcctt
     1021 tcgagctctg cagcactctt atctacataa ggatgaaggt aatccggagt gcccaacttt
     1081 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     1141 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc
     1201 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     1261 ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa
     1321 tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     1381 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     1441 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     1501 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     1561 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     1621 gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga
     1681 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     1741 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     1801 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     1861 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     1921 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     1981 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     2041 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     2101 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     2161 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     2221 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     2281 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     2341 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     2401 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca
     2461 cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     2521 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     2581 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2641 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     2701 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     2761 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     2821 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2881 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2941 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3001 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3061 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3121 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3181 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     3241 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     3301 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     3361 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     3421 tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg
     3481 gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg
     3541 ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg
     3601 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt
     3661 atttgatgcc tggcagttcc ctactctcgc gttaacgct
//
