LOCUS       sequence-165381-        3870 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing MAPK14, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_MAPK14_WT
FEATURES             Location/Qualifiers
     source          1..3870
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      51..639
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     540..559
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     793..810
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(969..996)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1088..1174)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1223..1245
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1237..1254
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1238..1254
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1270..1369
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             2360..2368
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(2450..2549)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2566..2585)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2567..2585)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2590..2606)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2590..2606)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3037..3828
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
       61 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
      121 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
      181 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
      241 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
      301 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
      361 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
      421 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
      481 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
      541 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
      601 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
      661 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
      721 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
      781 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
      841 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
      901 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta
      961 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
     1021 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
     1081 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     1141 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
     1201 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag
     1261 ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
     1321 gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagttggcat gtctcaggag
     1381 aggcccacgt tctaccggca ggagctgaac aagacaatct gggaggtgcc cgagcgttac
     1441 cagaacctgt ctccagtggg ctctggcgcc tatggctctg tgtgtgctgc ttttgacaca
     1501 aaaacggggt tacgtgtggc agtgaagaag ctctccagac catttcagtc catcattcat
     1561 gcgaaaagaa cctacagaga actgcggtta cttaaacata tgaaacatga aaatgtgatt
     1621 ggtctgttgg acgtttttac acctgcaagg tctctggagg aattcaatga tgtgtatctg
     1681 gtgacccatc tcatgggggc agatctgaac aacattgtga aatgtcagaa gcttacagat
     1741 gaccatgttc agttccttat ctaccaaatt ctccgaggtc taaagtatat acattcagct
     1801 gacataattc acagggacct aaaacctagt aatctagctg tgaatgaaga ctgtgagctg
     1861 aagattctgg attttggact ggctcggcac acagatgatg aaatgacagg ctacgtggcc
     1921 actaggtggt acagggctcc tgagatcatg ctgaactgga tgcattacaa ccagacagtt
     1981 gatatttggt cagtgggatg cataatggcc gagctgttga ctggaagaac attgtttcct
     2041 ggtacagacc atattgatca gttgaagctc attttaagac tcgttggaac cccaggggct
     2101 gagcttttga agaaaatctc ctcagagtct gcaagaaact atattcagtc tttgactcag
     2161 atgccgaaga tgaactttgc gaatgtattt attggtgcca atcccctggc tgtcgacttg
     2221 ctggagaaga tgcttgtatt ggactcagat aagagaatta cagcggccca agcccttgca
     2281 catgcctact ttgctcagta ccacgatcct gatgatgaac cagtggccga tccttatgat
     2341 cagtcctttg aaagcaggga cctccttata gatgagtgga aaagcctgac ctatgatgaa
     2401 gtcatcagct ttgtgccacc accccttgac caagaagaga tggagtcctg cccaactttc
     2461 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     2521 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
     2581 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     2641 tacattgcac aagataaaaa tatatcatca tgcctcctct agaccagcca ggacagaaat
     2701 gcctcgactt cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag
     2761 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     2821 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     2881 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     2941 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     3001 cagcagggca gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa
     3061 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
     3121 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt
     3181 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
     3241 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
     3301 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     3361 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     3421 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     3481 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     3541 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     3601 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
     3661 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
     3721 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
     3781 tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac
     3841 ccatgaccaa aatcccttaa cgtgagttac
//
