LOCUS       sequence-165382-        4038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CSNK1E, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CSNK1E_WT
FEATURES             Location/Qualifiers
     source          1..4038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      61..649
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     550..569
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     803..820
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(979..1006)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1098..1184)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1233..1255
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1247..1264
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1248..1264
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1280..1379
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2628..2727)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2744..2763)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2745..2763)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2768..2784)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2768..2784)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3215..4006
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
       61 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
      121 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      181 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      241 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      301 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      361 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      421 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      481 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      541 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      601 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      661 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      721 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      781 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      841 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      901 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga
      961 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc
     1021 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc
     1081 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
     1141 aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc
     1201 tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc
     1261 cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt
     1321 gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagttggcat
     1381 ggagctacgt gtggggaaca agtaccgcct gggacggaag atcgggagcg ggtccttcgg
     1441 agatatctac ctgggtgcca acatcgcctc tggtgaggaa gtcgccatca agctggagtg
     1501 tgtgaagaca aagcaccccc agctgcacat cgagagcaag ttctacaaga tgatgcaggg
     1561 tggcgtgggg atcccgtcca tcaagtggtg cggagctgag ggcgactaca acgtgatggt
     1621 catggagctg ctggggccta gcctcgagga cctgttcaac ttctgttccc gcaaattcag
     1681 cctcaagacg gtgctgctct tggccgacca gatgatcagc cgcatcgagt atatccactc
     1741 caagaacttc atccaccggg acgtcaagcc cgacaacttc ctcatggggc tggggaagaa
     1801 gggcaacctg gtctacatca tcgacttcgg cctggccaag aagtaccggg acgcccgcac
     1861 ccaccagcac attccctacc gggaaaacaa gaacctgacc ggcacggccc gctacgcttc
     1921 catcaacacg cacctgggca ttgagcaaag ccgtcgagat gacctggaga gcctgggcta
     1981 cgtgctcatg tacttcaacc tgggctccct gccctggcag gggctcaaag cagccaccaa
     2041 gcgccagaag tatgaacgga tcagcgagaa gaagatgtca acgcccatcg aggtcctctg
     2101 caaaggctat ccctccgaat tctcaacata cctcaacttc tgccgctccc tgcggtttga
     2161 cgacaagccc gactactctt acctacgtca gctcttccgc aacctcttcc accggcaggg
     2221 cttctcctat gactacgtct ttgactggaa catgctgaaa ttcggtgcag cccggaatcc
     2281 cgaggatgtg gaccgggagc ggcgagaaca cgaacgcgag gagaggatgg ggcagctacg
     2341 ggggtccgcg acccgagccc tgccccctgg cccacccacg ggggccactg ccaaccggct
     2401 ccgcagtgcc gccgagcccg tggcttccac gccagcctcc cgcatccagc cggctggcaa
     2461 tacttctccc agagcgatct cgcgggtcga ccgggagagg aaggtgagta tgaggctgca
     2521 caggggtgcg cccgccaacg tctcctcctc agacctcact gggcggcaag aggtctcccg
     2581 gatcccagcc tcacagacaa gtgtgccatt tgaccatctc gggaagtgcc caactttctt
     2641 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     2701 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     2761 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     2821 cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg acagaaatgc
     2881 ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc
     2941 acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg
     3001 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     3061 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
     3121 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     3181 gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga tcgccgaagt
     3241 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     3301 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
     3361 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     3421 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     3481 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     3541 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     3601 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     3661 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     3721 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     3781 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     3841 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     3901 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     3961 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct cgagccaccc
     4021 atgaccaaaa tcccttaa
//
