LOCUS       sequence-165383-        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into MFE1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Schwartz C, Shabbir-Hussain M, Frogue K, Blenner M, Wheeldon I
  TITLE     Standardized markerless gene integration for pathway engineering in 
            Yarrowia lipolytica.
  JOURNAL   ACS Synth Biol. 2016 Dec 19.
  PUBMED    27989123
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Dec 19."
COMMENT     Sequence Label: pHR_MFE1_hrGFP
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gaccatgatt acgccaagct ttgaagagct cgagcatctc ctcagtgaag acagaagcag
       61 tcatggcagt accagcgtta ggggcaatgg tgttgacaag aatgttgtac ttctcaccct
      121 ctcgagcaag agctcgggag aaaccgagga taccagcctt ggcggcagag tagttggcct
      181 ggccgaagtt accgtagata ccagaagttg aggtggtgtt gatgacacgg ccgtacttct
      241 gcttaaggaa gtggggccac gcggccttgg taacggagta agtaccgttg aggtggacat
      301 caaagatcag ctgccacatc tcatcatcca tgttggcgaa agacttgtct cggagaatac
      361 cagcgttgtt gacaacggcg tggacagcac cgaaggcgtc gatagcggtc tgaacaacct
      421 tctcaccgtg gatgacgttg ttcttgtcag cgacggcgat accaccgagg gccttaattt
      481 catcgacaac cttctgaggg ttaccgaaat cgttaacaac gaccttggca ccaagcttag
      541 caaggaggtg agagtaagct cggccaatgc cagcaccggc tccagtgaca atgacaacct
      601 ggtccttgaa ggagacggta ggtccctggg ggttctcggg tcgcttaaca gactcctcag
      661 cgtatttgaa gaagtcagca gggccgttag ggtaggtggg gctctcaaaa gaggtgacct
      721 catcccatcg cttcagaata gcagacgggg tgaaagtgtc gtcgcccttg aaaacagcac
      781 cgttgcctcg ctcccatcgg attttagcca tgtaaccagc accgacctcg taaataccat
      841 aagactcggt gacagagtcg tgggtgaggt agccgaccag aggaacaacg tactcaggct
      901 tgaggagctt gaggatatcc tcgggcatga ctgtctcggt cattcgggaa gcagcaagag
      961 gagcgatgac gttggaagta atgttgtact tggcaccctc cttggcgaga gtctcaccga
     1021 acctagggca aattaaagcc ttcgagcgtc ccaaaacctt ctcaag
//
