LOCUS       sequence-165384-        6555 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ERBB2, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6555)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 6555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_ERBB2_WT
FEATURES             Location/Qualifiers
     source          1..6555
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      57..645
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     546..565
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     799..816
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(975..1002)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1094..1180)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1229..1251
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1243..1260
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1244..1260
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1276..1375
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(5141..5240)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5257..5276)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5258..5276)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5281..5297)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5281..5297)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5728..6519
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
       61 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
      121 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
      181 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
      241 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      301 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      361 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      421 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      481 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
      541 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
      601 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      661 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
      721 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
      781 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
      841 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
      901 gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag
      961 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
     1021 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
     1081 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
     1141 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
     1201 ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt
     1261 cttaagctcg ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa
     1321 caaattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagt tggcaccatg
     1381 gagctggcgg ccttgtgccg ctgggggctc ctcctcgccc tcttgccccc cggagccgcg
     1441 agcacccaag tgtgcaccgg cacagacatg aagctgcggc tccctgccag tcccgagacc
     1501 cacctggaca tgctccgcca cctctaccag ggctgccagg tggtgcaggg aaacctggaa
     1561 ctcacctacc tgcccaccaa tgccagcctg tccttcctgc aggatatcca ggaggtgcag
     1621 ggctacgtgc tcatcgctca caaccaagtg aggcaggtcc cactgcagag gctgcggatt
     1681 gtgcgaggca cccagctctt tgaggacaac tatgccctgg ccgtgctaga caatggagac
     1741 ccgctgaaca ataccacccc tgtcacaggg gcctccccag gaggcctgcg ggagctgcag
     1801 cttcgaagcc tcacagagat cttgaaagga ggggtcttga tccagcggaa cccccagctc
     1861 tgctaccagg acacgatttt gtggaaggac atcttccaca agaacaacca gctggctctc
     1921 acactgatag acaccaaccg ctctcgggcc tgccacccct gttctccgat gtgtaagggc
     1981 tcccgctgct ggggagagag ttctgaggat tgtcagagcc tgacgcgcac tgtctgtgcc
     2041 ggtggctgtg cccgctgcaa ggggccactg cccactgact gctgccatga gcagtgtgct
     2101 gccggctgca cgggccccaa gcactctgac tgcctggcct gcctccactt caaccacagt
     2161 ggcatctgtg agctgcactg cccagccctg gtcacctaca acacagacac gtttgagtcc
     2221 atgcccaatc ccgagggccg gtatacattc ggcgccagct gtgtgactgc ctgtccctac
     2281 aactaccttt ctacggacgt gggatcctgc accctcgtct gccccctgca caaccaagag
     2341 gtgacagcag aggatggaac acagcggtgt gagaagtgca gcaagccctg tgcccgagtg
     2401 tgctatggtc tgggcatgga gcacttgcga gaggtgaggg cagttaccag tgccaatatc
     2461 caggagtttg ctggctgcaa gaagatcttt gggagcctgg catttctgcc ggagagcttt
     2521 gatggggacc cagcctccaa cactgccccg ctccagccag agcagctcca agtgtttgag
     2581 actctggaag agatcacagg ttacctatac atctcagcat ggccggacag cctgcctgac
     2641 ctcagcgtct tccagaacct gcaagtaatc cggggacgaa ttctgcacaa tggcgcctac
     2701 tcgctgaccc tgcaagggct gggcatcagc tggctggggc tgcgctcact gagggaactg
     2761 ggcagtggac tggccctcat ccaccataac acccacctct gcttcgtgca cacggtgccc
     2821 tgggaccagc tctttcggaa cccgcaccaa gctctgctcc acactgccaa ccggccagag
     2881 gacgagtgtg tgggcgaggg cctggcctgc caccagctgt gcgcccgagg gcactgctgg
     2941 ggtccagggc ccacccagtg tgtcaactgc agccagttcc ttcggggcca ggagtgcgtg
     3001 gaggaatgcc gagtactgca ggggctcccc agggagtatg tgaatgccag gcactgtttg
     3061 ccgtgccacc ctgagtgtca gccccagaat ggctcagtga cctgttttgg accggaggct
     3121 gaccagtgtg tggcctgtgc ccactataag gaccctccct tctgcgtggc ccgctgcccc
     3181 agcggtgtga aacctgacct ctcctacatg cccatctgga agtttccaga tgaggagggc
     3241 gcatgccagc cttgccccat caactgcacc cactcctgtg tggacctgga tgacaagggc
     3301 tgccccgccg agcagagagc cagccctctg acgtccatca tctctgcggt ggttggcatt
     3361 ctgctggtcg tggtcttggg ggtggtcttt gggatcctca tcaagcgacg gcagcagaag
     3421 atccggaagt acacgatgcg gagactgctg caggaaacgg agctggtgga gccgctgaca
     3481 cctagcggag cgatgcccaa ccaggcgcag atgcggatcc tgaaagagac ggagctgagg
     3541 aaggtgaagg tgcttggatc tggcgctttt ggcacagtct acaagggcat ctggatccct
     3601 gatggggaga atgtgaaaat tccagtggcc atcaaagtgt tgagggaaaa cacatccccc
     3661 aaagccaaca aagaaatctt agacgaagca tacgtgatgg ctggtgtggg ctccccatat
     3721 gtctcccgcc ttctgggcat ctgcctgaca tccacggtgc agctggtgac acagcttatg
     3781 ccctatggct gcctcttaga ccatgtccgg gaaaaccgcg gacgcctggg ctcccaggac
     3841 ctgctgaact ggtgtatgca gattgccaag gggatgagct acctggagga tgtgcggctc
     3901 gtacacaggg acttggccgc tcggaacgtg ctggtcaaga gtcccaacca tgtcaaaatt
     3961 acagacttcg ggctggctcg gctgctggac attgacgaga cagagtacca tgcagatggg
     4021 ggcaaggtgc ccatcaagtg gatggcgctg gagtccattc tccgccggcg gttcacccac
     4081 cagagtgatg tgtggagtta tggtgtgact gtgtgggagc tgatgacttt tggggccaaa
     4141 ccttacgatg ggatcccagc ccgggagatc cctgacctgc tggaaaaggg ggagcggctg
     4201 ccccagcccc ccatctgcac cattgatgtc tacatgatca tggtcaaatg ttggatgatt
     4261 gactctgaat gtcggccaag attccgggag ttggtgtctg aattctcccg catggccagg
     4321 gacccccagc gctttgtggt catccagaat gaggacttgg gcccagccag tcccttggac
     4381 agcaccttct accgctcact gctggaggac gatgacatgg gggacctggt ggatgctgag
     4441 gagtatctgg taccccagca gggcttcttc tgtccagacc ctgccccggg cgctgggggc
     4501 atggtccacc acaggcaccg cagctcatct accaggagtg gcggtgggga cctgacacta
     4561 gggctggagc cctctgaaga ggaggccccc aggtctccac tggcaccctc cgaaggggct
     4621 ggctccgatg tatttgatgg tgacctggga atgggggcag ccaaggggct gcaaagcctc
     4681 cccacacatg accccagccc tctacagcgg tacagtgagg accccacagt acccctgccc
     4741 tctgagactg atggctacgt tgcccccctg acctgcagcc cccagcctga atatgtgaac
     4801 cagccagatg ttcggcccca gcccccttcg ccccgagagg gccctctgcc tgctgcccga
     4861 cctgctggtg ccactctgga aaggcccaag actctctccc cagggaagaa tggggtcgtc
     4921 aaagacgttt ttgcctttgg gggtgccgtg gagaaccccg agtacttgac accccaggga
     4981 ggagctgccc ctcagcccca ccctcctcct gccttcagcc cagccttcga caacctctat
     5041 tactgggacc aggacccacc agagcggggg gctccaccca gcaccttcaa agggacacct
     5101 acggcagaga acccagagta cctgggtctg gacgtgccag tgccaacttt cttgtacaaa
     5161 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     5221 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     5281 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     5341 caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact
     5401 tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc
     5461 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc
     5521 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     5581 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     5641 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     5701 agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact
     5761 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     5821 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     5881 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     5941 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     6001 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     6061 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     6121 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     6181 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     6241 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     6301 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     6361 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     6421 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
     6481 tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca
     6541 aaatccctta acgtg
//
