LOCUS       sequence-165386-        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into MFE1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Schwartz C, Shabbir-Hussain M, Frogue K, Blenner M, Wheeldon I
  TITLE     Standardized markerless gene integration for pathway engineering in 
            Yarrowia lipolytica.
  JOURNAL   ACS Synth Biol. 2016 Dec 19.
  PUBMED    27989123
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Dec 19."
COMMENT     Sequence Label: pHR_MFE1_hrGFP
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcggtacccg cttttcgtag ataatggaat acaaatggat atccagagta tacacatgga
       61 tagtatacac tgacacgaca attctgtatc tctttatgtt aactactgtg aggcgttaaa
      121 tagagcttga tatataaaat gttacatttc acagtctgaa cttttgcaga ttacctaatt
      181 tggtaagata ttaattatga actgaaagtt gatggcatcc ctaaatttga tgaaaggggg
      241 atcccccggg ttcgaaggta ccaaggaagc atgcggtact ctacaacttt gaaatgatga
      301 cattttcgag aaaatgagaa caaccgaata tttgtactcc atattgatac ggaaaaattc
      361 acaatggaat cacacaagat agctctgaac aattgaggac ccttttctcc ctaaattcat
      421 tgtggttgcg gttgtattgc caacatggat tacgcccgcc cgatgacgag cgacttgctt
      481 gagccgaggc agagtctaaa tcgggacaac gaaaccataa accccatagg aagccaaagt
      541 gaccactgca tgaccccagt atataacctg ccccacccct ccatacttgc tgctttctag
      601 atcaagttat accggacctg ctaactcaat tgaggtagaa gatacgttat taacacaatc
      661 tatttattca acacacacac acaatgtctg gagaactaag atacgacgga aaggtcgtca
      721 ttgttaccgg tgccggtggc ggtctcggta aggcatacgc ccttttctac ggctctcgag
      781 gagcctctgt tgttgtcaac gatcttggtg gcgacttcaa gggcgacggt gcccaggctg
      841 gcagtggcaa gcgagtgagt atcattacaa gcgcagcgaa gcgaaacgac ccaaaacgac
      901 accacacaga aggataaact aacaccaggt tgccgatgtt gtcgtcgacg agattgtttc
      961 caagggaggc aaggctgttg ctaactacga ctctgtcgag aacg
//
