LOCUS       sequence-165390-        1078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Introduces DSB at MFE1 locus in Y. lipolytica.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Schwartz C, Shabbir-Hussain M, Frogue K, Blenner M, Wheeldon I
  TITLE     Standardized markerless gene integration for pathway engineering in 
            Yarrowia lipolytica.
  JOURNAL   ACS Synth Biol. 2016 Dec 19.
  PUBMED    27989123
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Dec 19."
COMMENT     Sequence Label: pCRISPRyl_MFE1
FEATURES             Location/Qualifiers
     source          1..1078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..26)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcttacacct ttcgcttctt cttggggtcg gctcgagagt cgccgcccag ctgagacagg
       61 tcgattcggg tctcgtacag gccggtgata gactggtgga tcagggtggc gtccagcacc
      121 tccttggtag aggtgtatcg ctttcggtcg atggtggtgt cgaagtactt gaaggcagcg
      181 ggggctccca ggttggtcag ggtgaacagg tgaatgatgt tctcagcctg ctctcgaatg
      241 ggcttgtctc ggtgcttgtt gtaagcagac agcaccttat ccaggttggc gtcagccagg
      301 atcactcgct tagagaactc agagatctgc tcgatgatct cgtccaggta gtgcttatgc
      361 tgctccacga agagctgctt ctgctcgtta tcctcgggag agcccttcag cttctcgtaa
      421 tgggaggcga ggtacaggaa gttgacgtac ttggagggca gggccagctc gtttcccttc
      481 tgcagctcgc cggcagaggc cagcattcgc tttcgtccgt tctccagctc gaacagagag
      541 tacttgggga gcttgataat cagatctttc ttcacctcct tgtatccctt ggcctccagg
      601 aagtcaatgg ggttcttctc gaaagaagat cgctccatga tggtaatgcc cagcagctcc
      661 ttcacagact tcagcttctt agacttgccc ttctccacct tggccaccac cagcacagag
      721 taagccacgg tgggagagtc gaaacctccg tacttcttgg ggtcccagtc cttctttcgg
      781 gcgatcagct tgtcagagtt tcgcttgggc aggatggact ccttagagaa acctccggtc
      841 tgcacctcgg tcttcttcac gatgttcacc tggggcatag acagcacctt tcgcacggtg
      901 gcgaagtctc gtcccttgtc ccacacgatc tcaccggtct ctccgttggt ctcaatgagg
      961 ggtcgctttc gaatctcgcc gttagccagg gtaatctcgg tcttgaagaa gttcataatg
     1021 ttggagtaga agaagtactt ggcggtagcc ttgccaatct cctgctcgga cttggcaa
//
