LOCUS       sequence-165400-         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Introduces DSB at AXP locus in Y. lipolytica.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Schwartz C, Shabbir-Hussain M, Frogue K, Blenner M, Wheeldon I
  TITLE     Standardized markerless gene integration for pathway engineering in 
            Yarrowia lipolytica.
  JOURNAL   ACS Synth Biol. 2016 Dec 19.
  PUBMED    27989123
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Dec 19."
COMMENT     Sequence Label: pCRISPRyl_AXP
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(4..24)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttacaccttt cgcttcttct tggggtcggc tcgagagtcg ccgcccagct gagacaggtc
       61 gattcgggtc tcgtacaggc cggtgataga ctggtggatc agggtggcgt ccagcacctc
      121 cttggtagag gtgtatcgct ttcggtcgat ggtggtgtcg aagtacttga aggcagcggg
      181 ggctcccagg ttggtcaggg tgaacaggtg aatgatgttc tcagcctgct ctcgaatggg
      241 cttgtctcgg tgcttgttgt aagcagacag caccttatcc aggttggcgt cagccaggat
      301 cactcgctta gagaactcag agatctgctc gatgatctcg tccaggtagt gcttatgctg
      361 ctccacgaag agctgcttct gctcgttatc ctcgggagag cccttcagct tctcgtaatg
      421 ggaggcgagg tacaggaagt tgacgtactt ggagggcagg gccagctcgt ttcccttctg
      481 cagctcgccg gcagaggcca gcattcgctt tcgtccgttc tccagctcga acagagagta
      541 cttggggagc ttgataatca gatctttctt cacctccttg tatcccttgg cctccaggaa
      601 gtcaatgggg ttcttctcga aagaagatcg ctccatgatg gtaatgccca gcagctcctt
      661 cacagacttc agcttcttag acttgccctt ctccaccttg gccaccacca gcacagagta
      721 agccacggtg ggagagtcga aacctccgta cttcttgggg tcccagtcct tctttcgggc
      781 gatcagcttg tcagagtttc gcttgggcag gatggactcc ttagagaaac ctccggtctg
      841 cacctcggtc ttcttcacga tgttcacctg gggcatagac agcacctttc gcacggtggc
      901 gaagtctcgt cccttgtccc acacgatctc accggtctct ccgttggtct caatga
//
