LOCUS       sequence-165404-         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Introduces DSB at D17 locus in Y. lipolytica.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Schwartz C, Shabbir-Hussain M, Frogue K, Blenner M, Wheeldon I
  TITLE     Standardized markerless gene integration for pathway engineering in 
            Yarrowia lipolytica.
  JOURNAL   ACS Synth Biol. 2016 Dec 19.
  PUBMED    27989123
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Dec 19."
COMMENT     Sequence Label: pCRISPRyl_D17
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        512..587
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     637..655
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 acaaataggg gttccgcaca catttccccg aaaagtgcca cctgacgtcc ccagttgcaa
       61 aagttgacac aactctagat ctgcttccaa atatagaatc ataacaaggg ttagggtgtg
      121 attatataat attggtctta attgatgtgc tagggcttta aaagttggtt aaaataacgc
      181 tctaatgcct ttttaatata ttgtcttttt caaaatctca aatcggacac ttcttcgtgt
      241 atgagactcc attttttggc tccgtcacgt gatatgtatt atcagctata gtggtgtaaa
      301 caaagttttt tactagctgt aatggcattt tgtcggagtg gtaaatcgcc ttcttgttgt
      361 gcgttcgagt tctggactct gcactgggct actttgaaaa atacctctaa tgcgccgatg
      421 gtttagtggt aaaatccatc gttgccatcg atgggccccc ggttcgattc cgggtcggcg
      481 caggttgacg ttccgtaata taggtgacga cgttttagag ctagaaatag caagttaaaa
      541 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttttacgtc
      601 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccaga
      661 tcctctagag tcgaagcggc cgctatgtct gataaaagga tgtaacatag gcaagctgct
      721 cgtgagtgtt gagtacgaac cttagatcca aatcacccgc acccacggat atacttgctt
      781 gaatatacag tagtatgcgg ccgcttcgac accatatcat ataaaactaa caatgcattg
      841 aattaatacg aagactaccc gttgctatct ccacaccgtt atctccacgg tccaaaggct
      901 gctcaatgtg ctgcatacgt aac
//
