LOCUS       sequence-165410-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Introduces DSB at A08 locus in Y. lipolytica.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Schwartz C, Shabbir-Hussain M, Frogue K, Blenner M, Wheeldon I
  TITLE     Standardized markerless gene integration for pathway engineering in 
            Yarrowia lipolytica.
  JOURNAL   ACS Synth Biol. 2016 Dec 19.
  PUBMED    27989123
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Dec 19."
COMMENT     Sequence Label: pCRISPRyl_A08
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..31)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggctagctta cacctttcgc ttcttcttgg ggtcggctcg agagtcgccg cccagctgag
       61 acaggtcgat tcgggtctcg tacaggccgg tgatagactg gtggatcagg gtggcgtcca
      121 gcacctcctt ggtagaggtg tatcgctttc ggtcgatggt ggtgtcgaag tacttgaagg
      181 cagcgggggc tcccaggttg gtcagggtga acaggtgaat gatgttctca gcctgctctc
      241 gaatgggctt gtctcggtgc ttgttgtaag cagacagcac cttatccagg ttggcgtcag
      301 ccaggatcac tcgcttagag aactcagaga tctgctcgat gatctcgtcc aggtagtgct
      361 tatgctgctc cacgaagagc tgcttctgct cgttatcctc gggagagccc ttcagcttct
      421 cgtaatggga ggcgaggtac aggaagttga cgtacttgga gggcagggcc agctcgtttc
      481 ccttctgcag ctcgccggca gaggccagca ttcgctttcg tccgttctcc agctcgaaca
      541 gagagtactt ggggagcttg ataatcagat ctttcttcac ctccttgtat cccttggcct
      601 ccaggaagtc aatggggttc ttctcgaaag aagatcgctc catgatggta atgcccagca
      661 gctccttcac agacttcagc ttcttagact tgcccttctc caccttggcc accaccagca
      721 cagagtaagc cacggtggga gagtcgaaac ctccgtactt cttggggtcc cagtccttct
      781 ttcgggcgat cagcttgtca gagtttcgct tgggcaggat ggactcctta gagaaacctc
      841 cggtctgcac ctcggtcttc ttcacgatgt tcacctgggg catagacagc acctttcgca
      901 cggtggcgaa gtctcgtccc ttgtcccaca cgatctcacc ggtctctccg ttggtctcaa
      961 tga
//
