LOCUS       sequence-165425-        4317 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing LCK, part of the Target Accelerator 
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_LCK_WT
FEATURES             Location/Qualifiers
     source          1..4317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     387..406
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     640..657
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(816..843)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(935..1021)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1070..1092
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1084..1101
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1085..1101
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1117..1216
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2744..2843)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2860..2879)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2861..2879)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2884..2900)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2884..2900)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3331..4122
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(4215..4317,1..486)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
       61 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
      121 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
      181 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
      241 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
      301 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
      361 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
      421 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
      481 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
      541 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
      601 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
      661 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
      721 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg
      781 cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
      841 tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
      901 gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca
      961 ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt
     1021 gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga
     1081 cgttgtaaaa cgacggccag tcttaagctc gggccccaaa taatgatttt attttgactg
     1141 atagtgacct gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg
     1201 tacaaaaaag ttggcaccat gggctgtggc tgcagctcac acccggaaga tgactggatg
     1261 gaaaacatcg atgtgtgtga gaactgccat tatcccatag tcccactgga tggcaagggc
     1321 acgctgctca tccgaaatgg ctctgaggtg cgggacccac tgattaccta cgaaggctcc
     1381 aatccgccgg cttccccact gcaagacaac ctggttatcg ctctgcacag ctatgagccc
     1441 tctcacgacg gagatctggg ctttgagaag ggggaacagc tccgcatcct ggagcagagc
     1501 ggcgagtggt ggaaggcgca gtccctgacc acgggccagg aaggcttcat ccccttcaat
     1561 tttgtggcca aagcgaacag cctggagccc gaaccctggt tcttcaagaa cctgagccgc
     1621 aaggacgcgg agcggcagct cctggcgccc gggaacactc acggctcctt cctcatccgg
     1681 gagagcgaga gcaccgcggg atcgttttca ctgtcggtcc gggacttcga ccagaaccag
     1741 ggagaggtgg tgaaacatta caagatccgt aatctggaca acggtggctt ctacatctcc
     1801 cctcgaatca cttttcccgg cctgcatgaa ctggtccgcc attacaccaa tgcttcagat
     1861 gggctgtgca cacggttgag ccgcccctgc cagacccaga agccccagaa gccgtggtgg
     1921 gaggacgagt gggaggttcc cagggagacg ctgaagctgg tggagcggct gggggctgga
     1981 cagttcgggg aggtgtggat ggggtactac aacgggcaca cgaaggtggc ggtgaagagc
     2041 ctgaagcagg gcagcatgtc cccggacgcc ttcctggccg aggccaacct catgaagcag
     2101 ctgcaacacc agcggctggt tcggctctac gctgtggtca cccaggagcc catctacatc
     2161 atcactgaat acatggagaa tgggagtcta gtggattttc tcaagacccc ttcaggcatc
     2221 aagttgacca tcaacaaact cctggacatg gcagcccaaa ttgcagaagg catggcattc
     2281 attgaagagc ggaattatat tcatcgtgac cttcgggctg ccaacattct ggtgtctgac
     2341 accctgagct gcaagattgc agactttggc ctagcacgcc tcattgagga caacgagtac
     2401 acagccaggg agggggccaa gtttcccatt aagtggacag cgccagaagc cattaactac
     2461 gggacattca ccatcaagtc agatgtgtgg tcttttggga tcctgctgac ggaaattgtc
     2521 acccacggcc gcatccctta cccagggatg accaacccgg aggtgattca gaacctggag
     2581 cgaggctacc gcatggtgcg ccctgacaac tgtccagagg agctgtacca actcatgagg
     2641 ctgtgctgga aggagcgccc agaggaccgg cccacctttg actacctgcg cagtgtgctg
     2701 gaggacttct tcacggccac agagggccag taccagcctc agcctccaac tttcttgtac
     2761 aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc
     2821 agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac
     2881 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt
     2941 gcacaagata aaaatatatc atcatgcctc ctctagacca gccaggacag aaatgcctcg
     3001 acttcgctgc tacccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga
     3061 acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca
     3121 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
     3181 atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa
     3241 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
     3301 ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc cgaagtatcg
     3361 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc
     3421 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg
     3481 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt
     3541 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt
     3601 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga
     3661 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat
     3721 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg
     3781 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta
     3841 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg
     3901 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac
     3961 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct
     4021 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
     4081 cactacgtga aaggcgagat caccaaggta gtcggcaaat aaccctcgag ccacccatga
     4141 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     4201 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     4261 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tcttttt
//
