LOCUS       sequence-165426-        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  [Edit] Dual Fluorescent endogenous miRNA sensor. To insert the 
            complementary region of the 3'UTR of RFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Lemus-Diaz N, Boker KO, Rodriguez-Polo I, Mitter M, Preis J, Arlt M,
            Gruber J
  TITLE     Dissecting miRNA gene repression on single cell level with an 
            advanced fluorescent reporter system.
  JOURNAL   Sci Rep. 2017 Mar 24;7:45197. doi: 10.1038/srep45197.
  PUBMED    28338079
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-03-24"; volume: "7:45197. doi"; pages: " 
            10.1038/srep45197"
COMMENT     Sequence Label: p.UTA.3.0 Empty
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             95..814
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     polyA_signal    865..986
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(876..895)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     930..949
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gagcgaccct gcagcgaccc gcttaaaagc ttggcattcc ggtactgttg gtaaagccct
       61 aagaacatta agaagggccc gggatccacc ggtcatggtg agcaagggcg ccgagctgtt
      121 caccggcatc gtgcccatcc tgatcgagct gaatggcgat gtgaatggcc acaagttcag
      181 cgtgagcggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      241 caccaccggc aagctgcctg tgccctggcc caccctggtg accaccctga gctacggcgt
      301 gcagtgcttc tcacgctacc ccgatcacat gaagcagcac gacttcttca agagcgccat
      361 gcctgagggc tacatccagg agcgcaccat cttcttcgag gatgacggca actacaagtc
      421 gcgcgccgag gtgaagttcg agggcgatac cctggtgaat cgcatcgagc tgaccggcac
      481 cgatttcaag gaggatggca acatcctggg caataagatg gagtacaact acaacgccca
      541 caatgtgtac atcatgaccg acaaggccaa gaatggcatc aaggtgaact tcaagatccg
      601 ccacaacatc gaggatggca gcgtgcagct ggccgaccac taccagcaga atacccccat
      661 cggcgatggc cctgtgctgc tgcccgataa ccactacctg tccacccaga gcgccctgtc
      721 caaggacccc aacgagaagc gcgatcacat gatctacttc ggcttcgtga ccgccgccgc
      781 catcacccac ggcatggatg agctgtacaa gtgagctgcc gcgactctag agtcggggcg
      841 gccggccgct tcgagcagac atgataagat acattgatga gtttggacaa accacaacta
      901 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
      961 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttc
//
