LOCUS       sequence-165427-         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  [Edit] Dual Fluorescent endogenous miRNA sensor. To insert the 
            complementary region of the 3'UTR of RFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Lemus-Diaz N, Boker KO, Rodriguez-Polo I, Mitter M, Preis J, Arlt M,
            Gruber J
  TITLE     Dissecting miRNA gene repression on single cell level with an 
            advanced fluorescent reporter system.
  JOURNAL   Sci Rep. 2017 Mar 24;7:45197. doi: 10.1038/srep45197.
  PUBMED    28338079
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-03-24"; volume: "7:45197. doi"; pages: " 
            10.1038/srep45197"
COMMENT     Sequence Label: p.UTA.3.0 Empty
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(73..768)
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     promoter        complement(790..808)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(851..870)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 atgtaatgaa aataaagata ttttattgcg gccagcggcc gctctaggtt taaacgaatt
       61 cccgggctcg agttatctgt gccccagttt gctagggagg tcgcagtact tggccacagc
      121 catctcgtgc tgctcgacgt aggtctcttt gtcggcctcc ttgattcttt ccagtctgtg
      181 gtccacgaag tggaagccgg gcatcttgag gttcttagcg ggtttcttgg atctgtatgt
      241 ggtcttgaag gagcagtgca ggtagccccc gcccacgagc ttcagggcca tctggctgtg
      301 gcctctcagg ccgccgtcag cggggtacag catctcggtg ttggcctccc agccgcgtgt
      361 tttcttctgc atcacagggc cgttggatgg gaagttcacc ccgttgatct tgacgttgta
      421 gatgatgcag ccgttctgga agctggtgtc ctgggtagcg gtcagcacgc ccccgtcttc
      481 gtatgtggtg attctctccc atgtgaagcc ctcagggaag gactgcttaa agaagtcggg
      541 gatgccctgg gtgtggttga tgaaggcttt gctgccgtac atgaagctgg tagccaggat
      601 gtcgaaggcg aaggggagag ggccgccctc gaccaccttg atcttcatgg tctgggtgcc
      661 ctcgtagggc ttgccttcgc cctcggatgt gcacttgaag tggtggttgt tcacggtgcc
      721 ctccatgtac agcttcatgt gcatgttctc cttgatcagc tcgctcatgg tggcgaccgg
      781 tagcgctagc ctatagtgag tcgtattaag tactctagcc ttaagagctg taattgaact
      841 gggagtggac acctgtggag agaaaggcaa agtggatgtc agtaagacca ataggtgcct
      901 atcagaaacg caagagtctt ctctgtctcg acaagcccag tttctattgg tctccttaaa
      961 cctg
//
