LOCUS       sequence-165436-        1018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector expressing ALCAM-p2A-E2Crimson.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Park RJ, Wang T, Koundakjian D, Hultquist JF, Lamothe-Molina P, 
            Monel B, Schumann K, Yu H, Krupzcak KM, Garcia-Beltran W, 
            Piechocka-Trocha A, Krogan NJ, Marson A, Sabatini DM, Lander ES, 
            Hacohen N, Walker BD
  TITLE     A genome-wide CRISPR screen identifies a restricted set of HIV host 
            dependency factors.
  JOURNAL   Nat Genet. 2016 Dec 19. doi: 10.1038/ng.3741.
  PUBMED    27992415
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2016 Dec 19. doi: 10.1038/ng.3741."
COMMENT     Sequence Label: ALCAM-p2A-E2Crimson
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggtgtcgtga cgcgggttaa ttaagccacc atggagtcaa aaggtgcctc ttcctgcagg
       61 ctgctgttct gtttgctgat ttccgcaaca gtgttccgcc ccggactcgg atggtatacc
      121 gtgaattctg cttatggtga tacgattatc attccatgcc ggttggatgt tccacagaat
      181 cttatgttcg gaaaatggaa gtacgagaag cccgatggct ctcctgtttt tattgccttc
      241 cggtccagca caaaaaagag cgtgcagtac gacgacgtgc ctgagtacaa ggacaggctt
      301 aacctgtccg agaactacac tctgtccatc tcaaatgccc ggatttccga tgagaagcgg
      361 ttcgtatgca tgctggtaac cgaagataat gtattcgagg cgcccaccat cgtgaaagtg
      421 tttaaacaac cgtcaaagcc cgagatcgtt tcaaaagcac tcttcttgga gactgaacaa
      481 ctcaagaagt tgggggattg catcagtgag gacagctatc cggacggaaa catcacttgg
      541 tatcggaacg gcaaagttct ccacccgctg gagggcgctg tggtcatcat cttcaagaag
      601 gagatggacc ctgttacgca gctttacaca atgaccagca ccttggagta caaaactact
      661 aaagcagata tccagatgcc ttttacatgc tccgtcactt actacggacc ttcaggacag
      721 aagaccatcc attctgagca ggcggtattc gatatttact accccacaga acaggtaact
      781 atacaggtgc tcccccctaa aaacgccatc aaagagggag acaacatcac ccttaagtgt
      841 cttggaaatg gtaatcctcc gccagaagag tttctgtttt atcttcctgg acagcctgag
      901 ggcatcagaa gcagcaatac gtataccctg acagatgtcc ggagaaacgc tacaggcgat
      961 tacaagtgct cactgatcga caaaaagagc atgattgctt ccaccgcaat caccgtgc
//
