LOCUS       sequence-165441-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: MCM3APlong_pCSdest
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcac aagtttgtac
      121 aaaaaagcag gctccgcggc cgcccccttc accatgaacc caactaatcc tttcagtggg
      181 cagcagccta gtgctttttc ggcgtcttct agtaatgtag gaacacttcc atctaagccg
      241 ccatttcgat ttggtcaacc ttctcttttt ggacaaaaca gtaccttatc tgggaagagc
      301 tcgggatttt cacaggtatc cagctttcca gcgtcttctg gagtaagtca ttcctcttca
      361 gtgcaaacat tagggttcac ccaaacctca agtgttggac ccttttctgg acttgagcac
      421 acttccacct ttgtggctac ctctgggcct tcaagtttat ctgtgctggg aaacacagga
      481 tttagtttta aatcacccac cagtgttggg gctttcccaa gcacttctgc ttttggacaa
      541 gaagctggag aaatagtgaa ctctggtttt gggaaaacag aattcagctt taaacctctg
      601 gaaaatgcag tgttcaaacc aatactgggg gctgaatctg agccagagaa aacccagagc
      661 caaattgctt ctgggttttt tacattttcc cacccaatta gtagtgcacc tggaggcctg
      721 gcccctttct cttttcctca agtaacaagt agttcagcta ccacttcaaa ttttaccttt
      781 tcaaa
//
