LOCUS       sequence-165445-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a fluorescent granule marker, neuropeptide-Y, for 
            localization and exocytosis studies..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Gandasi NR, Vesto K, Helou M, Yin P, Saras J, Barg S
  TITLE     Survey of Red Fluorescence Proteins as Markers for Secretory Granule
            Exocytosis.
  JOURNAL   PLoS One. 2015 Jun 19;10(6):e0127801. doi: 
            10.1371/journal.pone.0127801. eCollection 2015.
  PUBMED    26091288
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-06-19"; volume: "10(6):e0127801. doi"; pages: " 
            10.1371/journal.pone.0127801. eCollection 2015"
COMMENT     Sequence Label: NPY-td-Orange2
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(158..865)
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
       61 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
      121 atctagagtc gcggccgctt taacctgtgc ctccgctctt gtacaactcg tccatgccgc
      181 cggtggagtg gcggccctcg gcgcgttcgt actgttccac gatggtgtag tcctcgttgt
      241 gggaggtgat gtccaacttg atgtcgacga tgtaggcgcc gggcagctgc acgggcttct
      301 tggccttgta ggtggtcttg acctcggagg tgtagtggcc gccgtccttc agcttcagcc
      361 tcatcttgat cttgcccttc agggcaccgt cctcggggta catccgctcg gaggaggcct
      421 cccagcccat ggtcttcttc tgcatcacgg ggccgtcgga ggggaagttg gtgccgcgca
      481 gcttcacctt gtagatgaac tcgccgtcct gcagggagga gtcctgggtc acggtcacca
      541 cgccgccgtc ctcgtagttc atcacgcgct cccacttgaa gccctcgggg aaggacagct
      601 tgaagtagtc ggggatgtcg gcggggtgct tcacgtaggc cttggagccg taggtgaaat
      661 gaggggacag gatgtcccag gcgaagggca gggggccacc cttggtcacc ttcagcttag
      721 cggtctgaaa gccctcgtag gggcggccct cgccctcgcc ctcgatctcg aactcgtggc
      781 cgttcacgga gccctccatg cgcaccttga agcgcatgaa ctccttgatg atggccatgt
      841 tattctcctc gcccttgctc accattccac ctgtcccgcc gcttccgccg gtagaggtac
      901 cgctcttgta caactcgtcc atgccgccgg tgg
//
