LOCUS       sequence-165446-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: KCNE2_pCSdest
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..31
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(407..431)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    475..609
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(499..518)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     553..572
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(722..742)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(722..740)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(761..777)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(761..777)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(774..796)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    785..801
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(809..815,816..833,834..838))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 aggatcacaa gtttgtacaa aaaagcaggc accatgtcta ctttatccaa tttcacacag
       61 acgctggaag acgtcttccg aaggattttt attacttata tggacaattg gcgccagaac
      121 acaacagctg agcaagaggc cctccaagcc aaagttgatg ctgagaactt ctactatgtc
      181 atcctgtacc tcatggtgat gattggaatg ttctctttca tcatcgtggc catcctggtg
      241 agcactgtga aatccaagag acgggaacac tccaatgacc cctaccacca gtacattgta
      301 gaggactggc aggaaaagta caagagccaa atcttgaatc tagaagaatc gaaggccacc
      361 atccatgaga acattggtgc ggctgggttc aaaatgtccc cctgaaaccc agctttcttg
      421 tacaaagtgg tgatcctctc gagcctctag aactatagtg agtcgtatta cgtagatcca
      481 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
      541 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
      601 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
      661 gaggtttttt aattcgcggc cgcggcgcca atgcattggg cccggtaccc agcttttgtt
      721 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
      781 gaaattgtta tccgctcaca attccacaca acatacgagc cgggagcata aagtgtaa
//
