LOCUS       sequence-165447-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: KCNE2_pCSdest
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    38..62
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(438..462)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(516..534)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(517..534)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 atacgactca ctatagttct agaggctcga gaggatcacc actttgtaca agaaagctgg
       61 gtttcagggg gacattttga acccagccgc accaatgttc tcatggatgg tggccttcga
      121 ttcttctaga ttcaagattt ggctcttgta cttttcctgc cagtcctcta caatgtactg
      181 gtggtagggg tcattggagt gttcccgtct cttggatttc acagtgctca ccaggatggc
      241 cacgatgatg aaagagaaca ttccaatcat caccatgagg tacaggatga catagtagaa
      301 gttctcagca tcaactttgg cttggagggc ctcttgctca gctgttgtgt tctggcgcca
      361 attgtccata taagtaataa aaatccttcg gaagacgtct tccagcgtct gtgtgaaatt
      421 ggataaagta gacatggtgc ctgctttttt gtacaaactt gtgatccttg aattcgaatc
      481 gatgggatcc tgcaaaaaga acaagtagct tgtattctat agtgtcacct aaatcaagct
      541 tgctccgacg tccccaggca gaatggcggt tccctaaacg agcattgctt atatagacct
      601 cccattaggc acgcctaccg cccatttacg tcaatggaac gcccatttgc gtcattgccc
      661 ctccccattg acgtcaatgg ggatgtactt ggcagccatc gcgggccatt taccgccatt
      721 gacgtcaatg ggagtactgc caatgtaccc tggcgtactt ccaatagtaa tgtacttgcc
      781 aagttactat taatagatat tgatgtactg ccaagtgggc catttaccgt cat
//
