LOCUS       sequence-165450-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector expressing RAP2A-p2A-E2Crimson.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Park RJ, Wang T, Koundakjian D, Hultquist JF, Lamothe-Molina P, 
            Monel B, Schumann K, Yu H, Krupzcak KM, Garcia-Beltran W, 
            Piechocka-Trocha A, Krogan NJ, Marson A, Sabatini DM, Lander ES, 
            Hacohen N, Walker BD
  TITLE     A genome-wide CRISPR screen identifies a restricted set of HIV host 
            dependency factors.
  JOURNAL   Nat Genet. 2016 Dec 19. doi: 10.1038/ng.3741.
  PUBMED    27992415
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2016 Dec 19. doi: 10.1038/ng.3741."
COMMENT     Sequence Label: RAP2A-p2A-E2Crimson
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             611..667
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 aggtgtcgtg acgcgggtta attaagccac catgcgcgag tacaaagtgg tggtgctggg
       61 ctcgggcggg gtaggcaaat ccgccctgac cgtgcagttc gtgaccggca ccttcatcga
      121 gaaatacgac cccaccatcg aggacttcta ccgcaaggag atcgaggtgg attcgtcgcc
      181 gtcggtgctg gagatcctgg acacggcggg caccgagcag ttcgcgtcca tgcgggacct
      241 gtacatcaag aacggccagg gcttcatcct cgtctacagc ctcgtcaacc agcagagctt
      301 ccaggacatc aagcccatgc gggaccagat catccgcgtg aagcggtatg agaaagtgcc
      361 agtcatcttg gttgggaaca aagtggacct ggaaagtgag agagaagtat cgtccagcga
      421 aggcagagcc cttgctgaag agtggggctg cccctttatg gaaacttccg ctaagagtaa
      481 aacaatggtg gacgaactct ttgcagaaat tgtgaggcag atgaactatg ctgctcagcc
      541 tgacaaagat gacccatgct gttctgcatg taacatacaa gcggccgcag cggccgcagg
      601 aggtggagga gctacaaatt tcagcctgct gaagcaggcc ggcgacgtgg aggagaaccc
      661 cggccccaat agcactgaga acgtcatcaa gcccttcatg cgcttcaagg tgcacatgga
      721 gggctccgtg aacggccacg agttcgagat cgagggcgtg ggcgagggca agccctacga
      781 gggcacccag accgccaagc tgcaagtgac caagggcggc cccctgccct tcgcctggga
      841 catcctgtcc ccccagttct tctacggctc caaggcgtac atcaagcacc ccgccgacat
      901 ccccgactac ctcaagcagt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
      961 cgaggacggc g
//
