LOCUS       sequence-165453-         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a fluorescent granule marker, neuropeptide-Y, for 
            localization and exocytosis studies..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Gandasi NR, Vesto K, Helou M, Yin P, Saras J, Barg S
  TITLE     Survey of Red Fluorescence Proteins as Markers for Secretory Granule
            Exocytosis.
  JOURNAL   PLoS One. 2015 Jun 19;10(6):e0127801. doi: 
            10.1371/journal.pone.0127801. eCollection 2015.
  PUBMED    26091288
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-06-19"; volume: "10(6):e0127801. doi"; pages: " 
            10.1371/journal.pone.0127801. eCollection 2015"
COMMENT     Sequence Label: NPY-pHTomato
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(139..846)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKG
                     EIKMRLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(219..242)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(417..436)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     686..704
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
       61 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
      121 tctagagtcg cggccgcttt acttgtacag ctcgtccatg ccgccggtgg agtggcggcc
      181 ctcggcgcgc tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccag
      241 cttgatgtcg gtcttgtagg cgccgggcag ctgcacgggc ttcttggcca tgtaggtggt
      301 cttgacctcg gcgtcgtagt ggccgccgtc cttcagcttc agcctcatct tgatctcgcc
      361 cttcagggcg ccgtcctcgg ggtacatccg ctcggtggag gcctcccagc ccatggtctt
      421 cttctgcatt acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat
      481 gaactcgccg tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa
      541 gttcatcacg cgctcccact tgaagccctc ggggaaggac agcttcaagt agtcggggat
      601 gtcggcgggg tgcttcacgt aggccttgga gccgtacgtg aactgagggg acaggatgtc
      661 ccaggcgaag ggcagggggc cgcccttggt caccttcagc ttggcggtct gggtgccctc
      721 gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc
      781 catgcgcacc ttgaagcgca tgaactcctt gatgatggcc atgttattct cctcgccctt
      841 gctcacgacc ggtggatccc gggcccgcgg taccgtccac attgcagggt cttcaagccg
      901 agttctggga acattttctg tgctttctct catcaa
//
