LOCUS       sequence-165457-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: WRB_pCSdest
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    38..62
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(591..615)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(669..687)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(670..687)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 atacgactca ctatagttct agaggctcga gaggatcacc actttgtaca agaaagctgg
       61 gtttcagctg aacggatgaa gcacaatagc gacaactttg ttacagacta aaatccaaca
      121 ggtaattcca acaccacctg ctactctagt aggaaaggct accaggcggt ctaggggggt
      181 tatccattta ctcggcacga cagccacagg gacagaataa tacttccaaa tgagtgagat
      241 catcagggca gcctgcaata cgtagaaagc gacacttatc acccatttta tcttggctaa
      301 ttgagctgtc cgagctttca catgggtttt gagcttatcc gtcatcttgt tgatctttct
      361 ttccagcctg gcatatctgg caaactcgtc catcatgttg actgtggaga gctcctgctt
      421 catgtcctgg atctccgctc tcatctgtga ctcctgctcc gcgtccttct gcagcaccct
      481 ggacatgaag gatgagaagg acgggaggag gatcctaaga acattgcatc caaacacgaa
      541 gctgagcacc agcaaccacg cccagtggtc ggccgcggct gagctcatgg tgcctgcttt
      601 tttgtacaaa cttgtgatcc ttgaattcga atcgatggga tcctgcaaaa agaacaagta
      661 gcttgtattc tatagtgtca cctaaatcaa gcttgctccg acgtccccag gcagaatggc
      721 ggttccctaa acgagcattg cttatataga cctcccatta ggcacgccta ccgcccattt
      781 acgtcaatgg aacgcccatt tgcgtcattg cccctcccca ttgacgtcaa tggggatgta
      841 cttggcagcc atcgcgggcc atttaccgcc attgacgtca atgggagtac tgccaatgta
      901 ccctggcgta cttccaatag taatgtactt g
//
