LOCUS       sequence-165466-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a fluorescent granule marker, neuropeptide-Y, for 
            localization and exocytosis studies..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Gandasi NR, Vesto K, Helou M, Yin P, Saras J, Barg S
  TITLE     Survey of Red Fluorescence Proteins as Markers for Secretory Granule
            Exocytosis.
  JOURNAL   PLoS One. 2015 Jun 19;10(6):e0127801. doi: 
            10.1371/journal.pone.0127801. eCollection 2015.
  PUBMED    26091288
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-06-19"; volume: "10(6):e0127801. doi"; pages: " 
            10.1371/journal.pone.0127801. eCollection 2015"
COMMENT     Sequence Label: NPY-mApple
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(139..849)
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(417..436)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      846..855
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
       61 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
      121 atctagagtc gcggccgctt acttgtacag ctcgtccatg ccgccggtgg agtggcggcc
      181 ctcggcgcgt tcgtactgtt ccacgatggt gtagtcctcg ttgtgggaca cgatgtccaa
      241 cttgatgtcg acgatgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt
      301 cttgacctcg gcggcgtagt ggccgccgtc cttcagcttc agcctcttct tgatctcgct
      361 cttcagggcg ccgtcctcgg ggtacatccg ctcctcggag gcctcccagc ccatggtctt
      421 cttctgcatt acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat
      481 gaacacgccg tcctgcaggg aggagtcctg gttaacgtga ataatgccgc cgtcctcgaa
      541 gttcatcacg cgctcccacc tgaagccctc ggggaaggac agcttgaagt agtcggggat
      601 gtcggctggg tgcttaatgt agaccttgga gccgtacatg aactgagggg acaggatgtc
      661 ccaggcgaag ggcagggggc cacccttggt caccttcagc ttagcggtct gaaaggcctc
      721 gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc
      781 catgtgcacc ttgaagcgca tgaactcctt gatgatggcc atgttattct cctcgccctt
      841 gctcaccatg gtggcgaccg gtggatcccg ggcccgcggt accgtccaca ttgcagggtc
      901 ttcaagccga gttctgggaa cattttctgt gctttctctc atcaagaggt ctgaaatcag
      961 tgtctctggg ctggatcgtt ttccatatct ctgcctggtg atgaggttga tgta
//
