LOCUS       sequence-165467-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of CASTOR2 I280A mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Chantranupong L, Scaria SM, Saxton RA, Gygi MP, Shen K, Wyant GA, 
            Wang T, Harper JW, Gygi SP, Sabatini DM
  TITLE     The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway.
  JOURNAL   Cell. 2016 Mar 24;165(1):153-64. doi: 10.1016/j.cell.2016.02.035. 
            Epub 2016 Mar 10.
  PUBMED    26972053
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.02.035"; journalName: "Cell"; date: 
            "2016-03-24- 24"; volume: "165"; issue: "1"; pages: "153-64"
COMMENT     Sequence Label: pLJC6-Flag CASTOR1 I280A
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      327..336
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(501..520)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 cacgtggtgc tcggccagtg gcgcgatgac cgaacgggcg atcttggtga ccccagcagc
       61 ctgcactgcc gcaccgctgt gagacgacac gttcagcacc agccatgtgg cctcagctac
      121 ttgcaggaac tcagatgggg gcagctcttt aaagccctcc tcgtccacca taagcgtgta
      181 atcctcaggg gtctccgtca ggctgaagaa cttgcaccgg ctgcggcggg gcaggaagag
      241 cagcttgatg agcgggtggg tgtagagcca gagaccggga cgggcgacgc tcagcacccg
      301 cacccggtgt tctaggatgt gcagctccat ggtggcaccg gtagcgctag cgcttcgtct
      361 aacaaaaaag ccaaaaacgg ccagaattta gcggacaatt tactagtcta acactgaaaa
      421 ttacatattg acccaaatga ttacatttca aaaggtgcct aaaaaacttc acaaaacaca
      481 ctcgccaacc ccgagcgcat agttcaaaac cggagcttca gctacttaag aagataggta
      541 cataaaaccg accaaagaaa ctgacgcctc acttatccct cccctcacca gaggtccggc
      601 gcctgtcgat tcaggagagc ctaccctagg cccgaaccct gcgtcctgcg acggagaaaa
      661 gcctaccgca cacctaccgg caggtggccc caccctgcat tataagccaa cagaacgggt
      721 gacgtcacga cacgacgagg gcgcgcgctc ccaaaggtac gggtgcactg cccaacggca
      781 ccgccataac tgccgccccc gcaacagacg acaaaccgag ttctccagtc agtgacaaac
      841 ttcacgtcag ggtccccaga tggtgcccca gcccatctca cccgaataag agctttcccg
      901 cattagcgaa ggcctcaaga ccttggggtt cttgccgccc cccatgcccc ccaccttggt
      961 tcaacgaact tcacagcccg ccttca
//
