LOCUS       sequence-16547-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD16_43
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             64..84
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(174..196)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     gap             618..627
                     /estimated_length=10
ORIGIN
        1 gctgcngcct gcaggtcgac tctagaggat ccccgggtac cgagctcaga aaaaatgact
       61 gctccaaaga agaagcgtaa ggtaccggtg ggtgaagacc agaaacagca cctcgaactg
      121 agccgcgata ttgcccagcg tttcaacgcg ctgtatggcg agatcgatcc cgtcgtttta
      181 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
      241 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
      301 cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa
      361 agctggctgg agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc aaactggcag
      421 atgcacggtt acgatgcgcc catctacacc aacgtgacct atcccattac ggtcaatccg
      481 ccgtttgttc ccacggagaa tccgacgggt tgttactcgc tcacatttaa tgttgatgaa
      541 agctggctac aggaaggcca gacgcgaatt atttttgatg gcgttaactc ggcgtttcat
      601 ctgtggtgca acgggggnnn nnnnnnntta ggcnnaggaa tntcgtttgc cgtnganttt
      661 tgacctgagc gcatttttac gcgccggaga aaaccgcctc gcggtgatgg tgctgcctgn
      721 atgagcgttt ntgaganaaa aaagantggg ggaaaangnt ttncggactt tttgnnaaaa
      781 caaaaaaaaa nattctgggc ccccttanna aanacccccc gangagtaat aagggnatct
      841 nnca
//
