LOCUS       sequence-165478-         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  individual 6N barcode to make barcoded cell line variants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Gruner BM, Schulze CJ, Yang D, Ogasawara D, Dix MM, Rogers ZN, 
            Chuang CH, McFarland CD, Chiou SH, Brown JM, Cravatt BF, Bogyo M, 
            Winslow MM
  TITLE     An in vivo multiplexed small-molecule screening platform.
  JOURNAL   Nat Methods. 2016 Sep 12. doi: 10.1038/nmeth.3992.
  PUBMED    27617390
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Sep 12. doi: 10.1038/nmeth.3992."
COMMENT     Sequence Label: MSCV-6NBC-GFP-PGK-Puro-Barcode70
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(41..43,44..46,47..760)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(41..43,44..46,47..757)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(86..107)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(347..366)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     694..715
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctccttctct aggcgccgga attagatccg ccaagccacc atggtgagca agggcgagga
       61 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
      121 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
      181 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
      241 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
      301 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
      361 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
      421 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
      481 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
      541 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
      601 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
      661 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
      721 cgccgggatc actctcggca tggacgagct gtacaagtag cggagaattc taccgggtag
      781 gggaggcgct ttt
//
