LOCUS       sequence-165479-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  individual 6N barcode to make barcoded cell line variants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Gruner BM, Schulze CJ, Yang D, Ogasawara D, Dix MM, Rogers ZN, 
            Chuang CH, McFarland CD, Chiou SH, Brown JM, Cravatt BF, Bogyo M, 
            Winslow MM
  TITLE     An in vivo multiplexed small-molecule screening platform.
  JOURNAL   Nat Methods. 2016 Sep 12. doi: 10.1038/nmeth.3992.
  PUBMED    27617390
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Sep 12. doi: 10.1038/nmeth.3992."
COMMENT     Sequence Label: MSCV-6NBC-GFP-PGK-Puro-Barcode71
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        97..596
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(132..155)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     529..548
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(617..636)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 cagccctcac tccttctcta ggcgccggaa ttagatcccc gcgtccacca tggtgagcaa
       61 gggcgacgag ctgtacaagt agcggagaat tctaccgggt aggggaggcg cttttcccaa
      121 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc
      181 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg
      241 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc
      301 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg
      361 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt
      421 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg
      481 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc
      541 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct
      601 gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
      661 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
      721 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
      781 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
//
