LOCUS       sequence-165491-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of mouse IL1a and mRFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Zhang B, Kwon OJ, Henry G, Malewska A, Wei X, Zhang L, Brinkley W, 
            Zhang Y, Castro PD, Titus M, Chen R, Sayeeduddin M, Raj GV, Mauck R,
            Roehrborn C, Creighton CJ, Strand DW, Ittmann MM, Xin L
  TITLE     Non-Cell-Autonomous Regulation of Prostate Epithelial Homeostasis by
            Androgen Receptor.
  JOURNAL   Mol Cell. 2016 Sep 15;63(6):976-89. doi: 
            10.1016/j.molcel.2016.07.025. Epub 2016 Sep 1.
  PUBMED    27594448
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.07.025"; journalName: "Mol Cell"; date: 
            "2016-09-15- 15"; volume: "63"; issue: "6"; pages: "976-89"
COMMENT     Sequence Label: FU-mILa-CRW
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(64..741)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(64..741)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(126..149)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(324..343)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     593..611
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      738..747
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(816..840)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(837..888)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(866..886)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tcaatctttc acaaattttg taatccagag gttgattatc gataagcttg atatcgaatt
       61 atcttaggcg ccagtggagt ggcggccctc ggcgcgctcg tactgttcca cgatggtgta
      121 gtcctcgttg tgggaggtga tgtccagctt gatgtcggtc ttgtaggcgc cgggcagctg
      181 cacgggcttc ttggccatgt aggtggtctt gacctcggcg tcgtagtggc cgccgtcctt
      241 cagcttcagc ctcatcttga tctcgccctt cagggcgccg tcctcggggt acatccgctc
      301 ggtggaggcc tcccagccca tggtcttctt ctgcattacg gggccgtcgg aggggaagtt
      361 ggtgccgcgc agcttcacct tgtagatgaa ctcgccgtcc tgcagggagg agtcctgggt
      421 cacggtcacc acgccgccgt cctcgaagtt catcacgcgc tcccacttga agccctcggg
      481 gaaggacagc ttcaagtagt cggggatgtc ggcggggtgc ttcacgtagg ccttggagcc
      541 gtactggaac tgaggggaca ggatgtccca ggcgaagggc agggggccgc ccttggtcac
      601 cttcagcttg gcggtctggg tgccctcgta ggggcggccc tcgccctcgc cctcgatctc
      661 gaactcgtgg ccgttcacgg agccctccat gcgcaccttg aagcgcatga actccttgat
      721 gacgtcctcg gaggaggcca tggtggcggc ggatcctcta gctagagtcg gtgtcttcta
      781 tggaggtcaa aacagcgtgg atggcgtctc caggcgatct gacggttcac taaacgagct
      841 ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag
      901 ttgttacgac attttggaaa gtcccgttga
//
