LOCUS       sequence-165492-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  YFP expressed from pJ23119 in the glpK locus with gentamicin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pGCG26
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     212..233
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(300..311)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(324..353)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     promoter        complement(405..439)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
ORIGIN
        1 tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg
       61 gcgaagcact gcaggccgta gccgaaggtg gtcacgaggg tgggccaggg cacgggcagc
      121 ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc
      181 tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc
      241 accaccccgg tgaacagctc ctcgcccttg ctcaccatcg aggctgctgc ctgcatatga
      301 cgaccttcga tatggccgct gctgtgatga tgatgatgat gatgatgatg atggcccatg
      361 gtatgtgtct cgtaaatagt atacgcgata gcgatgcact agtagctagc attataccta
      421 ggactgagct agctgtcaac tcgagctttt ttaaatgggt taaattaggt caaaaaagtg
      481 tgtttttagg ctcaaaagac atcatttagg gaggaatttc agataacaga aaaccttcgc
      541 cattgtgcaa gtgaatttgg gctaagtcat agatggtgtt gatcttcaaa gtcggtgtgg
      601 cgatcgccaa ctgccaacga tccaaatcca gggcgagcca tttgccaatc tcaacggtct
      661 tcggagcccc ggccatgtgc gccactaaca caagccgctg ctgggtgtcg ggattggtgc
      721 gcctgccgta gaaaatggtg cgttggtgat ccgaccaatg gtgcaggcga tcgccccttg
      781 caggatcgag atgatttcgc agccaaggct gggcctggcg atattgccgc aagcgtaaat
      841 taaagctcgc ccgctcatcc ggtaccgatc cacaataatg ggagacccga caagcctcgt
      901 ggccatcctc cataaaggcg atcgctattt c
//
