LOCUS       sequence-165493-       11395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of mouse IL1a and mRFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11395)
  AUTHORS   Zhang B, Kwon OJ, Henry G, Malewska A, Wei X, Zhang L, Brinkley W, 
            Zhang Y, Castro PD, Titus M, Chen R, Sayeeduddin M, Raj GV, Mauck R,
            Roehrborn C, Creighton CJ, Strand DW, Ittmann MM, Xin L
  TITLE     Non-Cell-Autonomous Regulation of Prostate Epithelial Homeostasis by
            Androgen Receptor.
  JOURNAL   Mol Cell. 2016 Sep 15;63(6):976-89. doi: 
            10.1016/j.molcel.2016.07.025. Epub 2016 Sep 1.
  PUBMED    27594448
REFERENCE   2  (bases 1 to 11395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.07.025"; journalName: "Mol Cell"; date: 
            "2016-09-15- 15"; volume: "63"; issue: "6"; pages: "976-89"
COMMENT     Sequence Label: FU-mILa-CRW
FEATURES             Location/Qualifiers
     source          1..11395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             815..995
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1042..1167
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1660..1893
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2078..2122
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2420..2537
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2602..3815
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3652..3671
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3875..3884
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        4763..5066
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5067..5270
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5267..5291
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5360..5369
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5366..6043
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             5366..6043
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(5496..5514)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5764..5783
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5958..5981
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    6070..6659
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6123..6143)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6541..6552)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7200..7380
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7406..7423)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7412..7636
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7682..8110
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7769..7788)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7979..8000
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8119..8139)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8124..8453
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8304..8439
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8501..8548
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8567..8941
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9071..9192
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9108..9127)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9162..9181
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9241..9257)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9241..9257)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9254..9276)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9265..9281
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9289..9295,9296..9313,9314..9319))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9334..9355
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9472..9489)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9643..10231)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9723..9742)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10402..11193,11194..11262))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11025..11044
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11263..11367)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctctg tactgggtct ctctggttag accagatctg
      841 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      901 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      961 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     1021 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     1081 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     1141 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     1201 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     1261 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     1321 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1381 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1441 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1501 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1561 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1621 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1681 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1741 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1801 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1861 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1921 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1981 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     2041 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     2101 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     2161 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     2221 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     2281 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     2341 agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa
     2401 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     2461 aaagaatagt agaaataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     2521 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggttaatta
     2581 acccgtgtcg gctccagatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc
     2641 ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt
     2701 ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt
     2761 atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc
     2821 cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc
     2881 cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc
     2941 cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg
     3001 tgagtagcgg gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac
     3061 ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg
     3121 aactgggggt tggggggagc gcacaaaatg gcggctgttc ccgagtcttg aatggaagac
     3181 gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga
     3241 acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg
     3301 gggcaccatc tggggacccc tgacgtgaag tttgtcactg actggagaaa ctcgggtttg
     3361 tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg
     3421 ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt
     3481 ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg
     3541 gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag
     3601 tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc
     3661 ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt
     3721 ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg
     3781 ctaaattctg gccgtttttg gcttttttgt tagacgaagc ttgggctgca ggtcgactct
     3841 agagctagcg ttaacgaatt cggcttatct agacgccacc atggccaaag ttcctgactt
     3901 gtttgaagac ctaaagaact gttacagtga aaatgaagac tacagttctg ccattgacca
     3961 tctctctctg aaccagaaat ccttctatga tgcaagctat ggctcacttc atgagacttg
     4021 cacagaccag tttgtatctc tgagaacctc tgaaacgtca aagatgtcca acttcacctt
     4081 caaggagagc cgggtgacag tatcagcaac gtcaagcaac gggaagattc tgaagaagag
     4141 acggctgagt ttcagtgaga ccttcactga agatgacctg cagtccataa cccatgatct
     4201 ggaagagacc atccaaccca gatcagcacc ttacacctac cagagtgatt tgagatacaa
     4261 actgatgaag ctcgtcaggc agaagtttgt catgaatgat tccctcaacc aaactatata
     4321 tcaggatgtg gacaaacact atctcagcac cacttggtta aatgacctgc aacaggaagt
     4381 aaaatttgac atgtatgcct actcgtcggg aggagatgac tctaaatatc ctgttactct
     4441 aaaaatctca gattcacaac tgttcgtgag cgctcaagga gaagaccagc ccgtgttgct
     4501 gaaggagttg ccagaaacac caaaactcat cacaggtagt gagaccgacc tcattttctt
     4561 ctggaaaagt atcaactcta agaactactt cacatcagct gcttatccag agctgtttat
     4621 tgccaccaaa gaacaaagtc gggtgcacct ggcacgggga ctgccctcta tgacagactt
     4681 ccagatatca taaaagcagc cttatttcgg gagtctatga attcattgag tgcgtccgga
     4741 tccgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     4801 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     4861 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     4921 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     4981 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     5041 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     5101 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     5161 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     5221 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
     5281 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccgac
     5341 tctagctaga ggatccgccg ccaccatggc ctcctccgag gacgtcatca aggagttcat
     5401 gcgcttcaag gtgcgcatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     5461 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg
     5521 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc cagtacggct ccaaggccta
     5581 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     5641 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     5701 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     5761 cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc ggatgtaccc
     5821 cgaggacggc gccctgaagg gcgagatcaa gatgaggctg aagctgaagg acggcggcca
     5881 ctacgacgcc gaggtcaaga ccacctacat ggccaagaag cccgtgcagc tgcccggcgc
     5941 ctacaagacc gacatcaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
     6001 acagtacgag cgcgccgagg gccgccactc cactggcgcc taagataatt cgatatcaag
     6061 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6121 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6181 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6241 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6301 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     6361 cccctcccta ttgccacggc ggaactcatc gcccgcctgc cttgcccgct gctggacagg
     6421 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     6481 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtcct
     6541 tcggccctca atccaagcgg accttccttc ccgcggcctg ctgccggctc tgcgggcctc
     6601 ttccgcgtct ttcgccttcg ccctcagacg agtcggatct ccctttgggc gctccccgca
     6661 tcgatgtcga cctcgagacc ggccgaactc gaagacctag aaaaaacatt ggagcaatca
     6721 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     6781 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     6841 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     6901 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     6961 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca
     7021 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct
     7081 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga
     7141 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg
     7201 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7261 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7321 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7381 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     7441 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     7501 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     7561 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     7621 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     7681 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     7741 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     7801 acgttcgccg gctttccccg tcaagctcta aatcggggca tccctttagg gttccgattt
     7861 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     7921 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     7981 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     8041 taagggattt tggggatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     8101 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     8161 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     8221 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     8281 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     8341 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     8401 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     8461 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
     8521 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac
     8581 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
     8641 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
     8701 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
     8761 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
     8821 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
     8881 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg
     8941 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     9001 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     9061 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     9121 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     9181 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     9241 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     9301 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     9361 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     9421 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9481 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9541 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9601 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9661 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9721 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9781 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
     9841 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9901 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9961 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    10021 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    10081 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    10141 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    10201 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    10261 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    10321 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    10381 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    10441 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10501 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10561 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10621 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10681 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    10741 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10801 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10861 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10921 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10981 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    11041 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    11101 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    11161 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    11221 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    11281 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    11341 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgac
//
