LOCUS       sequence-165494-         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA targeting YFP expressed from pJ23119 in the NS1 locus with 
            kanamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas5
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(64..93)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_RNA        complement(114..189)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(211..245)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
ORIGIN
        1 aacagccaag ctggagaccg tttaaactca atgatgatga tgatgatggt cgacggcgct
       61 attcagatcc tcttctgaga tgagtttttg ttcgggccca agcttcaaaa aaagcaccga
      121 ctcggtgcca ctttttcaag ttgataacgg actagcctta ttttaacttg ctatttctag
      181 ctctaaaacg cgagggcgag ggcgatgcca gctagcatta tacctaggac tgagctagct
      241 gtcaagaatt ccgttattga tacaaaatta actcaagaaa aatagtgtga actaaaagat
      301 acagtaggat attttaatac agcaatttca tgcctatttg acgaatttct aagtatagta
      361 ataaaaacac ttggagtcag taaaaaaacg actgtgtttt agtgctttag aatgcaaaga
      421 caggcgtcac tgtaagctga agatttttta gccgctcttt ttttattttc atatagcctt
      481 tgagatattt ttcctcacta ttcaaaatat tatatttact cagtttttaa gtaattagca
      541 gacaattcta catcatcatc tagcttattg tgagtcgatt cattttgtcg ggcttcattt
      601 gtgctagaag cccgtccttg tgaaaccaac cgaactgcag aacattgccg cgccagtact
      661 ggataaacct ctggatctac ttgaatgcct gaagaaatca tgtcagcaca aagtttgact
      721 gtttccgctg aagattggat ttgaaacttt gctaattcgg cttgttctct ttccagtagg
      781 gtagtgctga aatctctaca gttgctgccc acttgaccac cgaggggcac aataatggca
      841 ccggaaagag cgtaggaatt tgaactctcc ccatcaaatg tattgccatt tgcagagcca
      901 ctgagcacaa aggaagggcg gggacaaacg atccctctaa aaccgtagg
//
