LOCUS       sequence-165495-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA targeting YFP expressed from pJ23119 in the NS1 locus with 
            kanamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas5
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agggatggag aaagcaaaat gaataaactg tttacagcag cccttagtgg tctatcattt
       61 atcgttgttc aacaggcaaa aagtgaacct gtatttattg attttgatta ccagtatcgg
      121 tatggttatg aatataatta cgactatgat gactatacct attactacca cgccaatgtc
      181 tacacctata gcgatgattc ggttgtattc ccagtcaaag tactcgttaa tgctgcatgt
      241 caacaaccac aataccccat tgtattaggc tctgaagcag atataagcaa cggtacagat
      301 gtcttgatcg aagaatctaa tgtcattatt gataatgatt tcagtacaaa ttgctctgct
      361 tttacagaaa aaaatgtcct gctactctgg gaagaaaacg agataaaata ggcgtccctt
      421 ctggtttttc tttctgtaaa gctgtactgt ttaaaaatag atcttaaatt atagaagcac
      481 tttgacttta ttttcggatc caccttttcg gggaaatgtg cgcggaaccc ctatttgttt
      541 atttttctaa atacattcaa atatgtatcc gctcatgaat taattcttag aaaaactcat
      601 cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa
      661 aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat
      721 cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct
      781 cgtcaaaaat aaggttatca agt
//
