LOCUS       sequence-165496-         285 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bidirectional tetracycline-regulated viral reporter that measures 
            the effects of 3' UTR sequences on mRNA and protein production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Zhao W, Pollack JL, Blagev DP, Zaitlen N, McManus MT, Erle DJ
  TITLE     Massively parallel functional annotation of 3' untranslated regions.
  JOURNAL   Nat Biotechnol. 2014 Apr;32(4):387-91. doi: 10.1038/nbt.2851. Epub 
            2014 Mar 16.
  PUBMED    24633241
REFERENCE   2  (bases 1 to 285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2851"; 
            journalName: "Nat Biotechnol"; date: "2014-04"; volume: "32"; issue:
            "4"; pages: "387-91"
COMMENT     Sequence Label: BTV
FEATURES             Location/Qualifiers
     source          1..285
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    27..45
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    69..87
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    111..129
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    153..171
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    195..213
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    237..255
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ggagtggtaa actcgacttt cacttttctc tatcactgat agggagtggt aaactcgact
       61 ttcacttttc tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac
      121 tgatagggag tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac
      181 tcgactttca cttttctcta tcactgatag ggagtggtaa actcgacttt cacttttctc
      241 tatcactgat agggagtggt aaactcgacc tatataagca gagct
//
