LOCUS       sequence-16551-e        1302 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L4043
FEATURES             Location/Qualifiers
     source          1..1302
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             667..687
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             709..729
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             751..771
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             793..813
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(856..884)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gttacatgat cttactaact aactattctc atttaaattt tcagagctta aaaatggctg
       61 aaatcactca caacgatgga tacgctaaca acttggaaat gaaataagct tgcatgcctg
      121 caggtcgact ctagaggatc aagagcattt gaatcagaat atggagaacg gagcatgagc
      181 attttcgaag ttttttagat gcactagaac aaagcgtgtt ggcttcctct gagcccgctt
      241 tccttatata cccgcattct gcagccttac agaatgttct agaaggtcct agatgcattc
      301 gtttgaaaat actcccggtg ggtgcaaaga gacgcagacg gaaaatgtat ctgggtctct
      361 ttaatgtgta cactactttt ccatgtaccg aatgtgagtc gccctccttt tgcaacaagc
      421 agctcgaatg ttctagaaaa aggtggaaaa tagtataaat accgttgaaa ataaataccg
      481 aacaacattt gctctaattg tgaaattaga aatcttcaaa ctataatcat ctcactggat
      541 cctatcgatt cgcggccgct gtacacccca aaggacccaa aggtatgttt cgaatgatac
      601 taacataaca tagaacattt tcaggaggac ccttggaggg taccgagctc agaaaaaatg
      661 actgctccaa agaagaagcg taaggtaccg gcagaaaaaa tgactgctcc aaagaagaag
      721 cgtaaggtac cggcagaaaa aatgactgct ccaaagaaga agcgtaaggt accggcagaa
      781 aaaatgactg ctccaaagaa gaagcgtaag gtaccggtag aaaaaatgag taaaggagaa
      841 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
      901 aaattttctg tcagtggaga agggtgaagg tgatgcaaca tacggaaaac ttacccctta
      961 aattttattt gcnctactgg aaaaactacc tgttccntgg ggtaagtttt aaaccatata
     1021 tatactaacc tancccngat natttnaatt ttccgccnac nccttgtcnc tactttcngg
     1081 ttangggggt tccanggctt cccngaaana cccanatcat atgaaacggc cttgacnttt
     1141 tncaagaang ccntgncccn aanggttatn tacnggnaaa nanctnnttt ttncaaaaaa
     1201 ggacgggaaa ctacaaaacc cgtaagttta anggttccgg gaccnaccaa cccnanaaan
     1261 tttaattttc cggggcgaaa cncaatttga ngggaanccc ng
//
