LOCUS       sequence-165518-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Chain A of the habu snake venom FIX/FX binding protein with a 
            C-terminal polyhistidine-tag. Cloned into pIRESneo3 (Ampicillin) via
            5' NheI and 3' BamHI; IRES vector conferring neomycin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Robert Peters
  TITLE     rFIX/X-binding protein
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: rFIXX-bp chain A (IRESneo)
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      107..116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             581..604
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     intron          682..911
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     894..913
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatcgatatc tgcggcctag ctagccgcca ccatggggcg
      121 attcatcttc atgagcttcg gcttgctggt cgtggctgcc tccctaagag gaactggagc
      181 tgattgtctc tctggttggt cctcctatga agggcattgc tacaaggcct tcgaaaaata
      241 caagacctgg gaagatgcag agagggtctg cacggagcag gcgaagggcg cgcatctggt
      301 ctctatcgaa agctccggag aagcagactt tgtggcccag ctggtcactc agaacatgaa
      361 gagactcgat ttctatatct ggatcgggct gagggttcaa ggcaaagtca agcaatgcaa
      421 ctcggagtgg agcgatggct ccagcgtcag ttatgagaac tggattgaag cagaatccaa
      481 aacgtgtctt gggctggaaa aagagacaga ttttcgtaag tgggtcaata tttactgtgg
      541 acaacaaaat cctttcgtct gcgaggcagg aggaggcagc caccatcacc accatcatca
      601 ccattgagga tccgcggccg catagataac tgatccagtg tgctggaatt aattcgctgt
      661 ctgcgagggc cagctgttgg ggtgagtact ccctctcaaa agcgggcatg acttctgcgc
      721 taagattgtc agtttccaaa aacgaggagg atttgatatt cacctggccc gcggtgatgc
      781 ctttgagggt ggccgcgtcc atctggtcag aaaagacaat ctttttgttg tcaagcttga
      841 ggtgtggcag gcttgagatc tggccataca cttgagtgac aatgacatcc actttgcctt
      901 tctctccaca ggtgtc
//
