LOCUS       sequence-165525-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 with sgRNA targeting Exon 1 of VEGF-A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Yiu G, Tieu E, Nguyen AT, Wong B, Smit-McBride Z
  TITLE     Genomic Disruption of VEGF-A Expression in Human Retinal Pigment 
            Epithelial Cells Using CRISPR-Cas9 Endonuclease.
  JOURNAL   Invest Ophthalmol Vis Sci. 2016 Oct 1;57(13):5490-5497. doi: 
            10.1167/iovs.16-20296.
  PUBMED    27768202
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1167/iovs"; 
            journalName: "Invest Ophthalmol Vis Sci"; date: "2016-10-1- 1"; 
            volume: "57"; issue: "13"; pages: "5490-5497"
COMMENT     Sequence Label: LCV2 V-1
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             280..327
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             328..351
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             361..417
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ttctccaaga gagtgatcct ggccgacgct aatctggaca aagtgctgtc cgcctacaac
       61 aagcaccggg ataagcccat cagagagcag gccgagaata tcatccacct gtttaccctg
      121 accaatctgg gagcccctgc cgccttcaag tactttgaca ccaccatcga ccggaagagg
      181 tacaccagca ccaaagaggt gctggacgcc accctgatcc accagagcat caccggcctg
      241 tacgagacac ggatcgacct gtctcagctg ggaggcgaca agcgacctgc cgccacaaag
      301 aaggctggac aggctaagaa gaagaaagat tacaaagacg atgacgataa gggatccggc
      361 gcaacaaact tctctctgct gaaacaagcc ggagatgtcg aagagaatcc tggaccgacc
      421 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
      481 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
      541 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
      601 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
      661 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
      721 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
      781 tggttcctgg ccaccgtcgg agtctcgccc gaccaccagg gcaagggtct gggcagcgcc
      841 gtcgtgctcc ccggagtgga
//
