LOCUS       sequence-165528-         311 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 with sgRNA targeting Exon 1 of VEGF-A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Yiu G, Tieu E, Nguyen AT, Wong B, Smit-McBride Z
  TITLE     Genomic Disruption of VEGF-A Expression in Human Retinal Pigment 
            Epithelial Cells Using CRISPR-Cas9 Endonuclease.
  JOURNAL   Invest Ophthalmol Vis Sci. 2016 Oct 1;57(13):5490-5497. doi: 
            10.1167/iovs.16-20296.
  PUBMED    27768202
REFERENCE   2  (bases 1 to 311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1167/iovs"; 
            journalName: "Invest Ophthalmol Vis Sci"; date: "2016-10-1- 1"; 
            volume: "57"; issue: "13"; pages: "5490-5497"
COMMENT     Sequence Label: LCV2 V-1
FEATURES             Location/Qualifiers
     source          1..311
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        144..219
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta
       61 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca
      121 ccgggaggaa gagtagctcg ccggttttag agctagaaat agcaagttaa aataaggcta
      181 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttgaatt cgctagctag
      241 gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc
      301 acagtccccg a
//
