LOCUS       sequence-165530-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral construct to express dCas9 fused with Dnmt3a-P2A-tagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Liu XS, Wu H, Ji X, Stelzer Y, Wu X, Czauderna S, Shu J, Dadon D, 
            Young RA, Jaenisch R
  TITLE     Editing DNA Methylation in the Mammalian Genome.
  JOURNAL   Cell. 2016 Sep 22;167(1):233-247.e17. doi: 
            10.1016/j.cell.2016.08.056.
  PUBMED    27662091
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.08"; journalName: "Cell"; date: "2016-09-22- 
            22"; volume: "167"; issue: "1"; pages: "233-247.e17"
COMMENT     Sequence Label: Fuw-dCas9-Dnmt3a-P2A-tagBFP
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(41..742)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             complement(743..799)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(800..826)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gcttgatatc tcaattaagc ttgtgcccca
       61 gtttgctagg gaggtcgcag tatctggcca ctgccacctc gtgctgctcg acgtaggtct
      121 cgttgttggc ctccttgatt ctttccagtc tgtagtccac atagtagacg ccaggcatct
      181 tgaggttctt agcgggtttc ttggatctat atgtggtctt gatgtttgcg atcagatggc
      241 tcccgcccac gagcttcagg gccatgtcgt ttctgccttc caggccgccg tcagcggggt
      301 acagcgtctc ggtgaaggcc tcccagccga gtgttttctt ctgcatcaca gggccgttgg
      361 atgtgaagtt cacccctctg atcttgacgt tgtagatgag gcagccgtcc tggaggctgg
      421 tgtcctgggt agcggtcagc acgcccccgt cttcgtatgt ggtgactctc tcccatgtga
      481 agccctcagg gaaggactgc ttgaagaagt cggggatgcc ctgggtgtgg ttgatgaagg
      541 tcttgctgcc gtagaggaag ctagtagcca ggatgtcgaa ggcgaagggg agagggccgc
      601 cctcgaccac cttgattctc atggtctggg tgccctcgta gggcttgcct tcgccctcgg
      661 atgtgcactt gaagtgatgg ttgtccacgg tgccctccat gtacagcttc atgtgcatgt
      721 tctccttaat cagctcgctc ataggcccgg ggttttcttc aacatctcct gcttgcttta
      781 acagagagaa gttcgtggcg gcgtagtcgg ggacgtcgta ggggtatgcg aattccacac
      841 acgcaaaata ctccttcagc ggagcgaaga ggtggcggat gactggcacg ctccatgacc
      901 ggcccagcag tctctgcctc gcca
//
