LOCUS       sequence-165533-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral construct to express dCas9 fused with an inactive 
            catalytic domain of Tet1 (H1672Y, D1674A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Liu XS, Wu H, Ji X, Stelzer Y, Wu X, Czauderna S, Shu J, Dadon D, 
            Young RA, Jaenisch R
  TITLE     Editing DNA Methylation in the Mammalian Genome.
  JOURNAL   Cell. 2016 Sep 22;167(1):233-247.e17. doi: 
            10.1016/j.cell.2016.08.056.
  PUBMED    27662091
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.08"; journalName: "Cell"; date: "2016-09-22- 
            22"; volume: "167"; issue: "1"; pages: "233-247.e17"
COMMENT     Sequence Label: Fuw-dCas9-Tet1CD_IM
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..67
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             71..91
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             95..115
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aactcggatt gacctctcac agctcggagg ggatgaggga gctgatccaa aaaagaagag
       61 aaaggtagac ccaaagaaaa aacgaaaagt agatccgaaa aagaagagga aggtgggatc
      121 cctgcccacc tgcagctgtc ttgatcgagt tatacaaaaa gacaaaggcc catattatac
      181 acaccttggg gcaggaccaa gtgttgctgc tgtcagggaa atcatggaga ataggtatgg
      241 tcaaaaagga aacgcaataa ggatagaaat agtagtgtac accggtaaag aagggaaaag
      301 ctctcatggg tgtccaattg ctaagtgggt tttaagaaga agcagtgatg aagaaaaagt
      361 tctttgtttg gtccggcagc gtacaggcca ccactgtcca actgctgtga tggtggtgct
      421 catcatggtg tgggatggca tccctcttcc aatggccgac cggctataca cagagctcac
      481 agagaatcta aagtcataca atgggcaccc taccgacaga agatgcaccc tcaatgaaaa
      541 tcgtacctgt acatgtcaag gaattgatcc agagacttgt ggagcttcat tctcttttgg
      601 ctgttcatgg agtatgtact ttaatggctg taagtttggt agaagcccaa gccccagaag
      661 atttagaatt gatccaagct ctcccttaca tgaaaaaaac cttgaagata acttacagag
      721 tttggctaca cgattagctc caatttataa gcagtatgct ccagtagctt accaaaatca
      781 ggtggaatat gaaaatgttg cccgagaatg tcggcttggc agcaaggaag gtcgaccctt
      841 ctctggggtc actgcttgcc tggacttctg tgctcatccc ctaca
//
