LOCUS       sequence-165538-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dominant negative Rac1 under cumate-inducible promoter in Lentiviral
            vector. Also expresses RFP and puromycin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   MacKay JL, Kumar S
  TITLE     Simultaneous and independent tuning of RhoA and Rac1 activity with 
            orthogonally inducible promoters.
  JOURNAL   Integr Biol (Camb). 2014 Sep;6(9):885-94. doi: 10.1039/c4ib00099d.
  PUBMED    25044255
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Integr Biol (Camb)"; date: "2014-09"; volume: "6"; issue: "9"; 
            pages: "885-94"
COMMENT     Sequence Label: pCuo DN Rac1
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     622..641
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 agcaccggta taatcaaaca gaccagattg tctgtttgtt accggtgttt agtgaaccgg
       61 gcgcgcctca tatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
      121 gggaccgatc cagcctccgc ggtcactctc ttccgcatcg ctgtctgcga gggccagctg
      181 ttgggctcgc ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac
      241 ccgtcggcct ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg
      301 atcggaaaac ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac
      361 cgtggcgggc ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat
      421 gtaattaaag taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga
      481 gatccagctg ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat
      541 tgtcagtttc caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact
      601 tgagtgacaa tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag
      661 tttggtctag tcgactggat ccggtaccga attcatgcag gccatcaagt gtgtggtggt
      721 gggagacgga gctgtaggta aaaactgcct actgatcagt tacacaacca atgcatttcc
      781 tggagaatat atccctactg tctttgacaa ttattctgcc aatgttatgg tagatggaaa
      841 accggtgaat ctgggcttat gggatacagc tggacaagaa gattatgaca gattacgccc
      901 cctatcctat ccgcaaacag atgtgttctt aatttgcttt tcccttgtga gtcctgcatc
      961 atttgaaaa
//
