LOCUS       sequence-16554-e        1273 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1273)
  TITLE     Fire Lab C. Elegans Vector Kit 2005
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD132.49
FEATURES             Location/Qualifiers
     source          1..1273
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..71
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     55..71
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(141..157)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(142..158)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(869..888)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(870..888)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(898..915)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(898..914)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(907..929)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ggattacctc actaaaggga acaaaagctg gagctccacc gcggtggcgg ccgctctaga
       61 actagtggat ccaccggttc catggctagc cacgtgacgc gtggatcccc cgggctgcag
      121 gaattcgata tcaagcttat cgataccgtc gacctcgacg agacgccgag tctcccttac
      181 tgcaaaaatg agaggggagc gaggtggtct acgtctctgt ggcacctaac actatttcgg
      241 ggacgcgtcg ccaagcggtg gtggtgccag cctcacggaa aggcttgcgg ggcgccgcgc
      301 tcgtgtccct ccgtgctctg ctgcgtgtgc cttccgcttc tcgtctgtcc tctctctcag
      361 tgcgcggggg gaccgcggca aaaaagaata acgacgaagc gaccgaatga cccagtctct
      421 tcacttctct actttcgatc ctcctccttc agctactcct cccatgtcca tccatcctcc
      481 gccccatcac tcccagagac cctttcggtc actctttcca atagactcta ccacaatcgg
      541 tcggactcat cacacttacc tttcaaatat ctaaactatt cctgaatata ataaatctta
      601 tagtttttag attctagaca atttctagag ttttggttgg tttatgagtt tatgcttccg
      661 gcctgttccc tgagacctca agtgtgagtg tactattgat gcttcacacc tgggctctcc
      721 gggtaccagg acggtttgag cagatctttt tttctgtttt cacggggttt tttttcacac
      781 tgggagctag ttccaataaa atggtattcg gggaaaaggg tcaaacgatt attttcagaa
      841 gtcgaggggg ggcccggtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
      901 ggccgtcgtt ttacaacgtc gtgactgggn aaaaccctgg cgttacccaa cttaatcgcc
      961 ttgcagcaca tccccctttt cgccagctgg cgtaaaagcn aaaaggccgc acngatcgnc
     1021 cnttccaana gttgcgcanc ctgaatggcg aangggaanc gcctgtaacg gcgcatnanc
     1081 ccggnggggg ggnggttacc ncannggaac gntaanttgc aggncctaag nccgctcctt
     1141 tgnttnttcc tnctttcccc ngttngcggt ttncngnaaa nttaannggg gntccnttag
     1201 ggtccaatna ggtttangnc ccncccccaa aaatnataag gggaggttcc nnaggggccn
     1261 cccntnaaaa gtt
//
