LOCUS       sequence-165540-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dominant negative Rac1 under cumate-inducible promoter in Lentiviral
            vector. Also expresses RFP and puromycin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   MacKay JL, Kumar S
  TITLE     Simultaneous and independent tuning of RhoA and Rac1 activity with 
            orthogonally inducible promoters.
  JOURNAL   Integr Biol (Camb). 2014 Sep;6(9):885-94. doi: 10.1039/c4ib00099d.
  PUBMED    25044255
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Integr Biol (Camb)"; date: "2014-09"; volume: "6"; issue: "9"; 
            pages: "885-94"
COMMENT     Sequence Label: pCuo DN Rac1
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(209..736,737..739,740..787))
                     /codon_start=1
                     /product="dominant negative mutant of human Rac family 
                     small GTPase 1"
                     /label=Rac1 (T17N)
                     /translation="MQAIKCVVVGDGAVGKNCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     primer_bind     complement(841..860)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 aggcggagcc agtacacgac atcactttcc cagtttaccc cgcgccacct tctctaggca
       61 cccgttcaat tgccgacccc tccccccaac ttctcgggga ctgtgggcga tgtgcgctct
      121 gcccactgac gggcaccgga gcgatcgcag atccttgcgg ccgcggatcc gatttaaatt
      181 cgaattcgct agctctagat atctcgagtt acaacagcag gcattttctc ttcctcttct
      241 tcacgggagg cgggcagagg actgctcgga tcgcttcgtc aaacactgtc ttgaggcctc
      301 gctgtgtgag cgccgagcac tccaggtatt ttacagcacc aatctcctta gccatggcta
      361 gaccctgcgg ataggtgatg ggagtcagct tcttctcctt cagtttctcg atcgtgtctt
      421 tatcatccct aagatcaagt ttagttccca ctaggatgat gggagtgttg ggacagtggt
      481 gccgcacctc aggataccac tttgcacgga cattttcaaa tgatgcagga ctcacaaggg
      541 aaaagcaaat taagaacaca tctgtttgcg gataggatag ggggcgtaat ctgtcataat
      601 cttcttgtcc agctgtatcc cataagccca gattcaccgg ttttccatct accataacat
      661 tggcagaata attgtcaaag acagtaggga tatattctcc aggaaatgca ttggttgtgt
      721 aactgatcag taggcagttt ttacctacag ctccgtctcc caccaccaca cacttgatgg
      781 cctgcatgaa ttcggtaccg gatccagtcg actagaccaa acttggacct gggagtggac
      841 acctgtggag agaaaggcaa agtggatgtc attgtcactc aagtgtatgg ccagatcggg
      901 gccaggtgaa tatcaaa
//
