LOCUS       sequence-165542-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dominant negative Rac1 under cumate-inducible promoter in Lentiviral
            vector. Also expresses RFP and puromycin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   MacKay JL, Kumar S
  TITLE     Simultaneous and independent tuning of RhoA and Rac1 activity with 
            orthogonally inducible promoters.
  JOURNAL   Integr Biol (Camb). 2014 Sep;6(9):885-94. doi: 10.1039/c4ib00099d.
  PUBMED    25044255
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Integr Biol (Camb)"; date: "2014-09"; volume: "6"; issue: "9"; 
            pages: "885-94"
COMMENT     Sequence Label: pCuo DN Rac1
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             13..281
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      309..318
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctgagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
       61 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
      121 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
      181 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
      241 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
      301 acgatatcgc caccatggcc agctccgagg atgtcatcaa agagtttatg agatttaagg
      361 tcaagatgga gggaagcgtc aacggacacg agttcgagat tgagggagaa ggagaaggcc
      421 ggccttacga gggcacacaa accgctaagc tcaaggtcac aaaaggagga cccctcccct
      481 tctcctggga tattctgagc cctcagttcc agtacggaag caaagcctat gttaaacacc
      541 ctgccgacat ccctgactat ctgaagctct ccttccctga aggcttcaag tgggagagat
      601 tcatgaactt cgaggacgga ggcgtggtga cagtcacaca agatagcacc ctccaggacg
      661 gagagtttat ttataaggtg aaactcagag gaaccaactt cccctccgat ggccctgtca
      721 tgcaaaaaaa aacaatggga tgggaagcct ccaccgagag aatgtatcct gaggatggcg
      781 ctctgaaagg cgaaattaaa atgagactga aactcaaaga cggaggacac tacgatgccg
      841 aggtcaaaac aacctacaag gccaagaaac aagtgcagct gcctggcgcc tacatgactg
      901 atattaaact cgacattatc agccataatg ggggactaca ccatcgtgga acaatatga
//
