LOCUS       sequence-165543-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pET28a expressing codon optimized superfolder GFP with C-terminal 
            His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Peeler JC, Mehl RA
  TITLE     Site-specific incorporation of unnatural amino acids as probes for 
            protein conformational changes.
  JOURNAL   Methods Mol Biol. 2012;794:125-34. doi: 10.1007/978-1-61779-331-8_8.
  PUBMED    21956560
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2012"; volume: "794:125-34. doi"; pages: " 
            10.1007/978-1-61779-331-8_8"
COMMENT     Sequence Label: pET28a-sfGFP
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             724..741
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggttagca aaggtgaaga actgtttacc ggcgttgtgc cgattctggt ggaactggat
       61 ggtgatgtga atggccataa atttagcgtt cgtggcgaag gcgaaggtga tgcgaccaac
      121 ggtaaactga ccctgaaatt tatttgcacc accggtaaac tgccggttcc gtggccgacc
      181 ctggtgacca ccctgaccta tggcgttcag tgctttagcc gctatccgga tcatatgaaa
      241 cgccatgatt tctttaaaag cgcgatgccg gaaggctatg tgcaggaacg taccattagc
      301 ttcaaagatg atggcaccta taaaacccgt gcggaagtta aatttgaagg cgataccctg
      361 gtgaaccgca ttgaactgaa aggtattgat tttaaagaag atggcaacat tctgggtcat
      421 aaactggaat ataatttcaa cagccataat gtgtatatta ccgccgataa acagaaaaat
      481 ggcatcaaag cgaactttaa aatccgtcac aacgtggaag atggtagcgt gcagctggcg
      541 gatcattatc agcagaatac cccgattggt gatggcccgg tgctgctgcc ggataatcat
      601 tatctgagca cccagagcgt tctgagcaaa gatccgaatg aaaaacgtga tcatatggtg
      661 ctgctggaat ttgttaccgc cgcgggcatt acccacggta tggatgaact gtataaaggc
      721 agccaccatc atcatcacca ttaa
//
